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Electrical field manipulation of cancer cell behavior monitored by whole cell biosensing device

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Abstract

All living cells possess electrical characteristics and are thus responsive to, and even generate electric fields and currents. It has been shown that the electrical properties of cancer cells differ from normal proliferating cells, thus electric fields may induce differential effects in normal and cancer cells. Manipulation of these electrical properties may provide a powerful direct and/or adjuvant therapeutic option for cancer. A whole cell impedance-based biosensor to monitor the effects of a range of different frequencies (50 kHz–2 MHz) at low-intensity (<2 V/cm) on the growth rate of human SKOV3 ovarian cancer cells versus non-cancerous HUVECs is reported. Rapid real-time monitoring of the SKOV3 behavior was observed as the alternating electric fields were applied and the impedimetric response of the cells was recorded. The cells were also labeled with propidium iodide to examine morphological changes and cell viability with fluorescence microscopy with trypan blue for comparison. A noticeable decrease in the growth profile of the SKOV3 was observed with the application of 200 kHz alternating electric fields indicating specific inhibitory effects on dividing cells in culture in contrast to the HUVECs. The outcome of this research will improve our fundamental understanding of the behavior of cancer cells when exposed to alternating electric fields at specific frequencies and foster the development strategies and optimal parameters for alternating electric field therapies for clinical and drug delivery applications.

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Acknowledgments

This research and development project was supported by the grant NIH R15 ES021079-01, Young Inventor Award by W. H. Coulter Foundation to Cz Li, and the Research Foundation for Doctoral Programs in Universities under the State Ministry of Education of China (No. 20100191110032) to Zz Wu.

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Authors and Affiliations

  1. Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, 10555 West Flagler Street, Miami, FL, 33174, USA

    Evangelia Hondroulis & Chen-Zhong Li

  2. Department of Pathology and Clinical Laboratories, Miami Children’s Hospital, Miami, FL, USA

    Steven J. Melnick

  3. Research Center for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing, 100083, China

    Xueji Zhang

  4. Key Laboratory of Biorheological Science and Technology of the State Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, People’s Republic of China

    Ze-Zhi Wu

Authors
  1. Evangelia Hondroulis
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  2. Steven J. Melnick
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  3. Xueji Zhang
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  4. Ze-Zhi Wu
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  5. Chen-Zhong Li
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Corresponding author

Correspondence to Chen-Zhong Li.

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Hondroulis, E., Melnick, S.J., Zhang, X. et al. Electrical field manipulation of cancer cell behavior monitored by whole cell biosensing device. Biomed Microdevices 15, 657–663 (2013). https://doi.org/10.1007/s10544-013-9788-2

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  • DOI: https://doi.org/10.1007/s10544-013-9788-2

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