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Non-invasive Cellular Characterization Using Bioimpedance Sensing

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BioSensing, Theranostics, and Medical Devices

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Abstract

Bioimpedance spectroscopy is one of the widely established interdisciplinary techniques used in cancer research for exploring electrophysiological features. Bioimpedance provides a real time, label-free, highly sensitive, cost-effective, and in vitro monitoring of living cells. The present chapter focuses on development of bioimpedimetric technology for exploring the biophysical properties of cancerous and normal cells in a suitable microenvironment. The chapter describes the detailed design and fabrication of a bioimpedance sensor, along with the experimental measurement for measuring impedance behavior of living cells. Initially, a numerical simulation has been presented to investigate the electric field distribution among electrode–cell–media interface and thereby operating frequency range has been identified. Subsequently, a novel fragmental frequency analysis method has been demonstrated to estimate the electrical parameters of group of cells from the experimental impedance data. Finally, a simple but detailed analysis technique has been developed to extract the electrical properties of a single cell from the previously collected bioimpedance response of a colony of cells. The investigation presented in this chapter provides an alternative approach to theoretically calculate equivalent electrical parameters of group of cells on the electrode surface and helps to extract electrical properties of a single cell from the bioimpedance spectroscopy of a cell colony during cell culture.

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Author information

Authors and Affiliations

  1. Department of ECE, IIIT Naya Raipur, Raipur, India

    Debanjan Das

  2. School of Medical Science and Technology, IIT Kharagpur, Kharagpur, India

    Soumen Das

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  1. Debanjan Das
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  2. Soumen Das
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Correspondence to Soumen Das .

Editor information

Editors and Affiliations

  1. Department of Medical Devices, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, Telangana, India

    Vivek Borse

  2. Department of Bioscience and Biochemical Engineering, Indian Institute of Technology, Varanasi, Uttar pradesh, India

    Pranjal Chandra

  3. Department of Bioscience Bioengineering, Indian Institute of Technology Bombay, Mumbai, Maharashtra, India

    Rohit Srivastava

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Das, D., Das, S. (2022). Non-invasive Cellular Characterization Using Bioimpedance Sensing. In: Borse, V., Chandra, P., Srivastava, R. (eds) BioSensing, Theranostics, and Medical Devices. Springer, Singapore. https://doi.org/10.1007/978-981-16-2782-8_6

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