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Integrated siphon-based metering and sedimentation of whole blood on a hydrophilic lab-on-a-disk

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Abstract

In this paper, we present a novel and fully integrated centrifugal microfluidic “lab-on-a-disk” for rapid colorimetric assays in human whole blood. All essential steps comprising blood sampling, metering, plasma extraction and the final optical detection are conducted within t = 150 s in passive, globally hydrophilized structures which obviate the need for intricate local hydrophobic surface patterning. Our technology features a plasma extraction structure (V = 500 nL, CV < 5%) where the purified plasma (c RBC < 0.11%) is centrifugally separated, metered by an overflow and subsequently extracted by a siphon-based principle through a hydrophilic extraction channel into the detection chamber.

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Acknowledgement

The authors are grateful to the partial support by the Ministry of Science, Research and the Arts of the German federal state of Baden-Wuerttemberg (contract 24-720.431-1-7/2) and the good cooperation with Jobst-Technologies.

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Authors and Affiliations

  1. Laboratory for MEMS Applications, Department for Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 106, 79110, Freiburg, Germany

    J. Steigert, T. Brenner, M. Grumann, L. Riegger, S. Lutz & R. Zengerle

  2. HSG-IMIT, Wilhelm-Schickard-Straße 10, 78052, Villingen-Schwenningen, Germany

    R. Zengerle & J. Ducrée

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  1. J. Steigert
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  2. T. Brenner
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  3. M. Grumann
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  4. L. Riegger
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  5. S. Lutz
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  6. R. Zengerle
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Correspondence to J. Steigert.

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Steigert, J., Brenner, T., Grumann, M. et al. Integrated siphon-based metering and sedimentation of whole blood on a hydrophilic lab-on-a-disk. Biomed Microdevices 9, 675–679 (2007). https://doi.org/10.1007/s10544-007-9076-0

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  • DOI: https://doi.org/10.1007/s10544-007-9076-0

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