Abstract
A microfluidic device combined with the microwell array and optical tweezers was set up for cell manipulation, localization and cultivation. Yeast cells were manipulated by a 1,064 nm laser and transferred to microwell array as a demonstration. The flow velocities at which the yeast cell can be confined in microwells of different sizes are charactered. The simulation of the cell’s flow trace in the microwell at different flow velocities is consisting with our experiment result. And we also proved a trapping laser power of 0.30 W is harmless for yeast cell cultivation. As a simple approach, this method can push forward the cell cultivation, cell interaction and other cell biology or biomedical studies in microfluidic system.
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Acknowledgements
The authors would like to thank Dr Weiguo Bao (Université Paris–Sud, France) for helpful suggestions. C.X. Luo gratefully acknowledges financial support of his stay in France by the Ministère des Affaires Etrangères (Ambassade de France en Chine). This work is partially supported by the Department of Science and Technology in China, Chinese Natural Science Foundation.
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Luo, C., Li, H., Xiong, C. et al. The combination of optical tweezers and microwell array for cells physical manipulation and localization in microfluidic device. Biomed Microdevices 9, 573–578 (2007). https://doi.org/10.1007/s10544-007-9066-2
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DOI: https://doi.org/10.1007/s10544-007-9066-2