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Effects of heat shock and salinity on barley growth and stress-related gene transcription

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Biologia Plantarum

Abstract

The effects of a short (30 min) heat shock (HS) on plants subsequently grown under a salinity stress (SS, 200 mM NaCl) for 10 d were investigated in barley (Hordeum vulgare L.) cv. Tokak 157/37. The maximum temperature for HS allowing plant survival was 45 °C. The root length was significantly decreased by SS, whereas HS alone did not affect root growth. Interestingly, HS stimulated root elongation under SS. An osmotic adjustment was promoted in leaves by SS. On the contrary, HS increased the osmotic potential in leaves in the absence of SS, and partly counteracted the effect of SS in the HS+SS treatment. Cu/Zn-SOD, HvAPX, HvCAT2, HSP17, HSP18, and HSP90 were transcribed in leaves of HS-treated plants, but not in control plants. The HSP70 was constitutively transcribed in both the SS and control plants, but after HS, a shorter amplicon was also observed. The genes coding antioxidants, Cu/Zn-SOD, HvCAT2 and HvAPX, were differentially influenced by SS or HS+SS in the roots and leaves. In the roots, the mRNA content of BAS1, HvDRF1, HvMT2, and HvNHX1 increased after the HS treatment. In a recovery experiment in which plants were grown to maturity after HS and HS+SS stress exposure, the plant height increased and the time to maturity was reduced in comparison with SS. Our results show that HS could stimulate plant growth and reduce some of the negative effects of SS, and that it affected the transcription of several stress-related genes.

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Abbreviations

ABA:

abscisic acid

APX:

ascorbate peroxidase

α-TUB:

α-tubuline

BAS1:

2-cis-peroxiredoxin 1

CAT:

catalase

Cu/Zn-SOD:

copper-zinc superoxide dismutase

DRF1:

dehydration responsive factor-1

GST:

glutathione-S-transferase

HS:

heat shock

MT2:

methallothionein-like protein type 2

NHX1:

Na+/H+ antiporter 1

SS:

salt stress

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Authors and Affiliations

  1. Department of Crop and Environment Sciences, Harper Adams University, TF10 8NB, Newport, UK

    M. Faralli & F. Gürel

  2. Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, 34134, Istanbul, Turkey

    C. Lektemur

  3. Department of Agricultural, Food and Environmental Sciences, University of Perugia, I-06121, Perugia, Italy

    D. Rosellini

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  1. M. Faralli
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  2. C. Lektemur
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  3. D. Rosellini
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  4. F. Gürel
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Correspondence to F. Gürel.

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Acknowledgements. The authors thank Nazaret Poyraz for technical assistance. This work was supported by the Scientific Research Projects Coordination Unit of the Istanbul University (No. BAP 4712) and the Erasmus Exchange Program to M.F.

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Faralli, M., Lektemur, C., Rosellini, D. et al. Effects of heat shock and salinity on barley growth and stress-related gene transcription. Biol Plant 59, 537–546 (2015). https://doi.org/10.1007/s10535-015-0518-x

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  • DOI: https://doi.org/10.1007/s10535-015-0518-x

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