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Evaluation of amino acid profile in contrasting arsenic accumulating rice genotypes under arsenic stress

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Amino acids (AAs) play significant roles in metal binding, antioxidant defense, and signaling in plants during heavy metal stress. In the present study, the essential amino acids (EAAs), non-essential amino acids (NEAAs), as well as the enzymes of proline and cysteine biosynthetic pathways were studied in contrasting arsenic accumulating rice genotypes grown in hydroponic solutions with addition of arsenate (AsV) or arsenite (AsIII). Under a mild As stress, the total AAs content significantly increased in both the rice genotypes with a greater increase in a low As accumulating rice genotype (LAARG; IET-19226) than in a high As accumulating rice genotype (HAARG; BRG-12). At the equimolar concentration (10 μM), AsIII had a greater effect on EAAs than AsV. Conversely, AsV was more effective in inducing a proline accumulation than AsIII. Among NEAAs, As significantly induced the accumulation of histidine, aspartic acid, and serine. In contrast, a higher As concentration (50 μM) reduced the content of most AAs, the effect being more prominent during AsIII exposure. The inhibition of glutamate kinase activity was noticed in HAARG, conversely, serine acetyltransferase and cysteine synthase activities were increased which was positively correlated with the cysteine synthesis.

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Abbreviations

AA:

amino acid

Ala:

alanine

Arg:

arginine

Asp:

aspartic acid

BCAAs:

branched chain amino acids

CS:

cysteine synthase

Cys:

cysteine

EEAs:

essential amino acids

GK:

glutamate kinase

Glu:

glutamic acid

Gly:

glycine

HAARG:

high As accumulating rice genotype

His:

histidine

Ile:

isoleucine

LAARG:

low As accumulating rice genotype

Leu:

leucine

Lys:

lysine

Met:

methionine

NEEAs:

non essential amino acids

Phe:

phenylalanine

Pro:

proline

RDI:

recommended daily intake

SAT:

serine acetyl transferase

Ser:

serine

Thr:

threonine

Tyr:

tyrosine

Val:

valine

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Authors and Affiliations

  1. CSIR-National Botanical Research Institute, Lucknow, 226001, India

    A. Kumar, S. Dwivedi, D. Chakrabarty, S. Mallick, P. K. Trivedi & R. D. Tripathi

  2. Department of Environmental Science, B.B.A. University, Lucknow, 226025, India

    A. Kumar & R. P. Singh

  3. Rice Research Station, Department of Agriculture, Government of West Bengal, Chinsurah, Hooghly, 712102, India

    B. Adhikari

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  1. A. Kumar
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  2. S. Dwivedi
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  3. R. P. Singh
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  4. D. Chakrabarty
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  8. R. D. Tripathi
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Correspondence to R. D. Tripathi.

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Acknowledgements: The authors are thankful to the Director of the National Botanical Research Institute, CSIR, Lucknow for the facilities and for the financial support from the CSIR-network projects (INDEPTH). A. Kumar is thankful to the Council of Scientific and Industrial Research, New Delhi, India for the award of Senior Research Fellowship. The authors are thankful to Dr. S. Mishra, the Department of Biology, the University of Kontanz, Germany, for the linguistic improvement of the MS.

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Kumar, A., Dwivedi, S., Singh, R.P. et al. Evaluation of amino acid profile in contrasting arsenic accumulating rice genotypes under arsenic stress. Biol Plant 58, 733–742 (2014). https://doi.org/10.1007/s10535-014-0435-4

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  • DOI: https://doi.org/10.1007/s10535-014-0435-4

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