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Discovery of loci determining pre-harvest sprouting and dormancy in wheat and barley applying segregation and association mapping

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Biologia Plantarum

Abstract

Three wheat and two barley populations were studied in order to find loci responsible for dormancy and pre-harvest sprouting. A classical quantitative trait loci analysis was combined with an association mapping approach. Many quantitative trait loci and marker trait associations could be detected on all seven chromosome groups of wheat and on the chromosomes 2H, 3H, 5H, 6H, and 7H of barley. Especially, the known regions on chromosomes 3A and 4A for wheat and 5H for barley were confirmed. Putative functions could be found via a candidate homologues search and via expressed sequence tag annotation. On chromosome 3A, the viviparous1 gene is located which is associated to preharvest sprouting and dormancy. On chromosome 4A, a protein is detected which belongs to the aquaporin family. In barley, an association with the aleurain gene on chromosome 5H was found. The expression of aleurain is regulated by abscisic acid and gibberelic acid. An influence of both hormones on dormancy and pre-harvest sprouting is known. It can be concluded that dormancy and pre-harvest sprouting are very complex traits regulated by multigenes and/or quantitative trait loci.

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Abbreviations

ABA:

abscisic acid

AM:

association mapping

CS:

Chinese Spring

D10:

dormancy at 10 °C

D20:

dormancy at 20 °C

DArT:

diversity array technology

DI:

dormancy index

EST:

expressed sequence tag

GA:

gibberellins

GLM:

general linear model

IPK:

Leibniz Institute of Plant Genetics and Crop Plant Research

ITMI:

International Triticeae Mapping Initiative

LOD:

logarithm of odds

MLM:

mixed linear model

SOD:

superoxide dismutase

MTA:

marker trait association

NCBI:

National Centre for Biotechnology Information

OWB:

Oregon Wolfe barley

PHS:

pre-harvest sprouting

QTL:

quantitative trait loci

References

  • Agrama, H.A., Eizenga, G.C., Yan, W.: Association mapping of yield and its components in rice cultivars. — Mol. Breed. 19: 341–356, 2007.

    Article  Google Scholar 

  • Aranzana, M.J., Kim, S., Zhao, K., Bakker, E., Horton, M., Jakob, K., Lister, C., Molitor, J., Shindo, C., Tang, C., Toomajian, C., Traw, B., Zheng, H., Bergelson, J., Dean, C., Marjoram, P., Nordborg, M.: Genome-wide association mapping in Arabidopsis identifies previously known flowering time and pathogen resistance genes. — PLoS Genet 1: e60, 2005.

    Article  PubMed  Google Scholar 

  • Baek, K.-H., Skinner, D.Z., Ling, P., Chen, X.: Molecular structure and organization of the wheat genomic manganese superoxide dismutase gene. — Genome 49: 209–218, 2006.

    Article  PubMed  CAS  Google Scholar 

  • Benech-Arnold, R.L., Giallorenzi, M.C., Frank, J., Rodriguez, V.: Termination of hull-imposed dormancy in developing barley grains is correlated with changes in embryonic ABA levels and sensitivity. — Seed Sci. Res. 9: 39–47, 1999.

    Article  Google Scholar 

  • Bethke, P.C., Fath, A., Spiegel, Y.N., Hwang, Y., Jones, R.L.: Abscisic acid, gibberellin and cell viability in cereal aleurone. — Euphytica 126: 3–11, 2002.

    Article  CAS  Google Scholar 

  • Börner, A., Schumann, E., Fürste, A., Cöster, H., Leithold, B., Röder, M.S., Weber, W.E.: Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). — Theor. appl. Genet. 105: 921–936, 2002.

    Article  PubMed  Google Scholar 

  • Breseghello, F., Sorrells, M.E.: Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. — Genetics 172: 1165–1177, 2006.

    Article  PubMed  Google Scholar 

  • Chang, C., Zhang, H.-P., Zhao, Q.-X., Feng, J.-M., Si, H.-Q., Lu, J., Ma, C.-X.: Rich allelic variations of Viviparous-1A and their associations with seed dormancy/pre-harvest sprouting of common wheat. — Euphytica 179: 343–353, 2011.

    Article  CAS  Google Scholar 

  • Costa, J.M., Corey, A., Hayes, P.M., Jobet, C., Kleinhofs, A., Kopisch-Obusch, A., Kramer, S.F., Kudrna, D., Li, M., Riera-Lizarazu, O., Sato, K., Szucs, P., Toojinda, T., Vales, M.I., Wolfe, R.I.: Molecular mapping of the Oregon Wolfe barleys: a phenotypically polymorphic doubled-haploid population. — Theor. appl. Genet. 103: 415–424, 2001.

    Article  CAS  Google Scholar 

  • Crossa, J., Burgueno, J., Dreisigacker, S., Vargas, M., Herrera-Foessel S.A., Lillemo, M., Singh, R.P., Trethowan, R., Warburton, M., Franco, J., Reynolds, M., Crouch, J.H., Ortiz, R.: Association analysis of historical bread wheat germplasm using additive genetic covariance of relatives and population structure. — Genetics 177: 1889–1913, 2007.

    Article  PubMed  CAS  Google Scholar 

  • Flintham, J.E.: Different genetic components control coatimposed and embryo-imposed dormancy in wheat. — Seed Sci. Res. 10: 43–50, 2000.

    Article  Google Scholar 

  • Flintham, J., Adlam, R., Bassoi, M., Holdsworth, M., Gale, M.: Mapping genes for resistance to sprouting damage in wheat. — Euphytica 126: 39–45, 2002.

    Article  CAS  Google Scholar 

  • Foley, M.E., Fennimore, S.A.: Genetic basis for seed dormancy. — Seed Sci. Res. 8: 173–182, 1998.

    Article  Google Scholar 

  • Forrest, K.L., Bhave, M.: Physical mapping of wheat aquaporin genes. — Theor. appl. Genet. 120: 863–873, 2010.

    Article  PubMed  CAS  Google Scholar 

  • Francki, M.G., Walker, E., Crawford, A.C., Broughton, S., Ohm, H.W., Barclay, I., Wilson, R.E., McLean, R.: Comparison of genetic and cytogenetic maps of hexaploid wheat (Triticum aestivum L.) using SSR and DArT markers. — Mol. Genet. Genomics 281: 181–191, 2009.

    Article  PubMed  CAS  Google Scholar 

  • Ganal, M.W., Röder, M.S.: Microsatellite and SNP markers in wheat breeding. — In: Varshney, R.K., Tuberosa, R. (ed.): Genomics Assisted Crop Improvement. Vol. 2: Genomics Applications in Crops. Pp. 1–24. Springer, New York 2007.

    Chapter  Google Scholar 

  • Gao, W., Clancy, J.A., Han, F., Prada, D., Kleinhofs, A., Ullrich, S.E.: Molecular dissection of a dormancy QTL region near the chromosome 7 (5H) L telomere in barley. — Theor. appl. Genet. 107: 552–559, 2003.

    Article  PubMed  CAS  Google Scholar 

  • Gerjets, T., Scholefield, D., Foulkes, M.J., Lenton, J.R., Holdsworth, M.J.: An analysis of dormancy, ABA responsiveness, after-ripening and pre-harvest sprouting in hexaploid wheat (Triticum aestivum L.) caryopses. — J. exp. Bot. 61: 597–607, 2010.

    Article  PubMed  CAS  Google Scholar 

  • Groos, C., Gay, G., Perretant, M.-R., Gervais, L., Bernard, M., Dedryver, F., Charmet, G.: Study of the relationship between pre-harvest sprouting and grain color by quantitative trait loci analysis in a whitexred grain breadwheat cross. — Theor. appl. Genet. 104: 39–47, 2002.

    Article  PubMed  CAS  Google Scholar 

  • Han, F., Ullrich, S.E., Clancy, J.A., Romagosa, I.: Inheritance and fine mapping of a major barley seed dormancy QTL. — Plant Sci. 143: 113–118, 1999.

    Article  CAS  Google Scholar 

  • Higa, A., Hidaka, T., Minai, Y., Matsuoka, Y., Haga, M.: Active oxygen radicals induce peroxidase activity in rice blade tissues. — Biosci. Biotechnol. Biochem. 65: 1852–1855, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Hildebrandt, T.M., Grieshaber, M.K.: Three enzymatic activities catalyze the oxidation of sulfide to thiosulfate in mammalian and invertebrate mitochondria. — FEBS J. 275: 3352–3361, 2008.

    Article  PubMed  CAS  Google Scholar 

  • Huang, J., Zhao, X., Huihui, Y., Ouyang, Y., Wang, L., Zhang, Q.: The ankyrin repeat gene family in rice: genome-wide identification, classification and expression profiling. — Plant mol. Biol. 71: 207–226, 2009.

    Article  PubMed  CAS  Google Scholar 

  • Jansen, C., Korell, M., Eckey, C., Biednkopf, D., Kogel, K.-H.: Identification and transcriptional analysis of powdery mildew-induced barley genes. — Plant Sci. 168: 373–380, 2005.

    Article  CAS  Google Scholar 

  • Kato, K., Nakamura, W., Tabiki, T., Miura, H., Sawada, S.: Detection of loci controlling seed dormancy on group 4 chromosomes of wheat and comparative mapping with rice and barley genomes. — Theor. appl. Genet. 102: 980–985, 2001.

    Article  CAS  Google Scholar 

  • Kervinen, T., Peltonen, S., Teeri, T.H., Karjalainen, R.: Differential expression of phenylalanine ammonia-lyase genes in barley induced by fungal infection or elicitors. — New Phytol. 139: 293–300, 1998.

    Article  CAS  Google Scholar 

  • Kikuchi, S., Taketa, S., Ichii, M., Kawasaki, S.: Efficient fine mapping of the naked caryopsis gene (nud) by HEGS (High Efficiency Genome Scanning)/AFLP in barley. — Theor. appl. Genet. 108: 73–78, 2003.

    Article  PubMed  CAS  Google Scholar 

  • Kleinhofs, A., Kilian, A., Saghai Maroof, M., Biyashev, R., Hayes, P., Chen, F., Lapitan, N., Fenwick, A., Blake, T., Kanazin, V., Ananiev, E., Dahleen, L., Kudrna, D., Bollinger, J., Knapp, S., Liu, B., Sorrells, M., Heun, M., Franckowiak, J., Hovman, D., Skadsen, R., Stevenson, B.: A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. — Theor. appl. Genet. 86: 705–713, 1993.

    Article  CAS  Google Scholar 

  • Kraakman, A.T.W., Niks, R.E., Van den Berg, P.M., Stam, P., Van Eeuwijk, F.A.: Linkage disequilibrium mapping of yield and yield stability in modern spring barley cultivars. — Genetics 168: 435–446, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Kraakman, A.T.W., Martinez, F., Mussiraliev, B., van Eeuwijk, F.A., Niks, R.E.: Linkage disequilibrium mapping of morphological, resistance, and other agronomically relevant traits in modern spring barley cultivars. — Mol. Breed. 17: 41–58, 2006.

    Article  CAS  Google Scholar 

  • Kulwal, P., Ishikawa, G., Benscher, D., Feng, Z., Yu, L.-X., Jadhav, A., Mehetre, S., Sorrells, M.E.: Association mapping for pre-harvest sprouting resistance in white winter wheat. — Theor. appl. Genet. 125: 793–805, 2012.

    Article  PubMed  CAS  Google Scholar 

  • Kulwal, P.L., Kumar, N., Gaur, A., Khurana, P., Khurana, J.P., Tyagi, A.K., Balyan, H.S., Gupta, P.K.: Mapping of a major QTL for pre-harvest sprouting tolerance on chromosome 3A in bread wheat. — Theor. appl. Genet. 111: 1052–1059, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Kulwal, P.L., Mir, R.R., Kumar, S., Gupta, P.K.: QTL analysis and molecular breeding for seed dormancy and pre-harvest sprouting tolerance in bread wheat. — J. Plant Biol. 37: 59–74, 2010.

    Google Scholar 

  • Kuroda, M., Kiyosaki, T., Matsumoto, I., Misaka, T., Arai, S., Abe, K.: Molecular cloning, characterization, and expression of wheat cystatins. — Biosci. Biotechnol. Biochem. 65: 22–28, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Lane, B.G.: Oxalate oxidases and differentiating surface structure in wheat: germins. — Biochem. J. 349: 309–321, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Li, C., Ni, P., Francki, M., Hunter, A., Zhang, Y., Schibeci, D., Li, H., Tarr, A., Wang, J., Cakir, M., Yu, J., Bellgard, M., Lance, R., Appels, R.: Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison. — Funct. Integr. Genomics 4: 84–93, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Liu, S., Bai, G., Cai, S., Chen, C.: Dissection of genetic components of preharvest sprouting resistance in white wheat. — Mol. Breed. 27: 511–523, 2011.

    Article  Google Scholar 

  • Lohwasser, U., Röder, M.S., Börner, A.: QTL mapping of the domestication traits pre-harvest sprouting and dormancy in wheat (Triticum aestivum L.). — Euphytica 143: 247–249, 2005.

    Article  CAS  Google Scholar 

  • Mares, D., Rathjen, J., Mrva, K., Cheong, J.: Genetic and environmental control of dormancy in white-grained wheat (Triticum aestivum L.). — Euphytica 168: 311–318, 2009.

    Article  CAS  Google Scholar 

  • McFadden, E.S., Sears, E.R.: The genome approach in radical wheat breeding. — J. amer. Soc. Agron. 39: 1011–1026, 1947.

    Article  Google Scholar 

  • Mori, M., Uchino, N., Chono, M., Kato, K., Miura, H.: Mapping QTLs for grain dormancy on wheat chromosome 3A and the group 4 chromosomes, and their combined effect. — Theor. appl. Genet. 110: 1315–1323, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Munkvold, J.D., Tanaka, J., Benscher, D. Sorrells, M.E.: Mapping quantitative trait loci for preharvest sprouting resistance in white wheat. — Theor. appl. Genet. 119: 1223–1235, 2009.

    Article  PubMed  CAS  Google Scholar 

  • Nagel, M., Vogel, H., Landjeva, S., Buck-Sorlin, G., Lohwasser, U., Scholz, U., Börner, A.: Seed conservation in ex situ genebanks — genetic studies on longevity in barley. — Euphytica 170: 5–14, 2009.

    Article  CAS  Google Scholar 

  • Nelson, J.C.: QGENE: software for mapping — based genomic analysis and breeding. — Mol. Breed. 3: 239–245, 1997.

    Article  CAS  Google Scholar 

  • Neumann, K., Kobiljski, B., Dencic, S., Varshney, R.K., Börner, A.: Genome-wide association mapping: a case study in bread wheat (Triticum aestivum L.). — Mol. Breed. 27: 37–58, 2011.

    Article  Google Scholar 

  • Noda, K., Matsuura, T., Maekawa, M., Taketa, S.: Chromosomes responsible for sensitivity of embryo to abscisic acid and dormancy in wheat. — Euphytica 123: 203–209, 2002.

    Article  CAS  Google Scholar 

  • Ogbonnaya, F.C., Imtiaz, M., Ye, G., Hearnden, P.R., Hernandez, E., Eastwood, R.F., van Ginkel, M., Shorter, S.C., Winchester, J.M.: Genetic and QTL analyses of seed dormancy and preharvest sprouting resistance in wheat germplasm CN10955. — Theor. appl. Genet. 116: 891–902, 2008.

    Article  PubMed  CAS  Google Scholar 

  • Osa, M., Kato, K., Mori, M., Shindo, C., Torada, A., Miura, H.: Mapping QTLs for seed dormancy and the Vp1 homologue on chromosome 3A in wheat. — Theor. appl. Genet. 106: 1491–1496, 2003.

    PubMed  CAS  Google Scholar 

  • Pestsova, E.G., Börner, A., Röder, M.S.: Development of a set of Triticum aestivum — Aegilops tauschii introgression lines. — Hereditas 135: 139–143, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Pestsova, E., Börner, A., Röder, M.S.: Development and QTL assessment of Triticum aestivum-Aegilops tauschii introgression lines. — Theor. appl. Genet. 112: 634–647, 2006.

    Article  PubMed  Google Scholar 

  • Prada, D., Ullrich, S.E., Molina-Cano, J.L., Cistue, L., Clancy, J.A., Romagosa, I.: Genetic control of dormancy in a Triumph/Morex cross in barley. — Theor. appl. Genet. 109: 62–70, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Pulido, P., Dominguez, F., Cejudo, F.J.: A hydrogen peroxide detoxification system in the nucleus of wheat seed cells. — Plant Signal Behav. 4: 23–25, 2009.

    Article  PubMed  CAS  Google Scholar 

  • Rehman Arif, M.A.: Seed longevity and dormancy in wheat (Triticum aestivum L.) — phenotypic variation and genetic mapping. — PhD Thesis, Martin-Luther-Universität Halle and Wittenberg Institut für Agrar- und Ernährungswissenschaften der Naturwissenschaftlichen Fakultät III, Halle 2012.

    Google Scholar 

  • Rehman Arif, M.A., Neumann, K., Nagel, M., Kobiljski, B., Lohwasser, U., Börner, A.: An association mapping analysis of dormancy and pre-harvest sprouting in wheat. — Euphytica 188: 409–417, 2012.

    Article  CAS  Google Scholar 

  • Romero, A., Alamillo, J.M., Garcia-Olmedo, F.: Processing of thionin precursors in barley leaves by a vacuolar proteinase. — Eur. J. Biochem. 243: 202–208, 1997.

    Article  PubMed  CAS  Google Scholar 

  • Sanchez-Monge, R., Delibes, A., Hernandez-Lucas, C., Carbonero, P., Garcia-Olmedo, F.: Homoeologous chromosomal location of the genes encoding thionins in wheat and rye. — Theor. appl. Genet. 54: 31–63, 1979.

    Article  Google Scholar 

  • Sato, K., Matsumoto, T., Ooe, N., Takeda, K.: Genetic analysis of seed dormancy QTL in barley. — Breed. Sci. 59: 645–650, 2009.

    Article  CAS  Google Scholar 

  • Stein, N., Prasad, M., Scholz, U., Thiel, T., Zhang, H.N., Wolf, M., Kota, R., Varshney, R.K., Perovic, D., Grosse, I., Graner, A.: A 1,000-loci transcript map of the barley genome: new anchoring points for integrative grass genomics. — Theor. appl. Genet. 114: 823–839, 2007.

    Article  PubMed  CAS  Google Scholar 

  • Strand, E.: Studies on seed dormancy in barley. — Meldinger fra Norges Landbrukshogskole Hoegskole 44: 1–23, 1965.

    Google Scholar 

  • Torada, A., Amano, Y.: Effect of seed coat color on seed dormancy in different environments. — Euphytica 126: 99–105, 2002.

    Article  CAS  Google Scholar 

  • Ullrich, S.E., Lee, H., Claney, J.A., Del Blanco, I.A., Jitkov, V.A., Kleinhofs, A., Han, F., Prada, D., Romagosa, I., Molina-Cano, J.L.: Genetic relationships between preharvest sprouting and dormancy in barley. — Euphytica 168: 331–345, 2009.

    Article  CAS  Google Scholar 

  • Xia, L.Q., Ganal, M.W., Shewry, P.R., He, Z.H., Yang, Y., Röder, M.S.: Exploiting the diversity of Viviparous-1 gene associated with pre-harvest sprouting tolerance in European wheat varieties. — Euphytica 159: 411–417, 2008.

    Article  CAS  Google Scholar 

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  1. Leibniz Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, D-06466, Gatersleben, Germany

    U. Lohwasser, M. A. Rehman Arif & A. Börner

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  1. U. Lohwasser
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  2. M. A. Rehman Arif
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  3. A. Börner
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Correspondence to U. Lohwasser.

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Acknowledgements: The authors thank Marion Röder and Nils Stein for providing the map data and Annette Marlow, Stefanie Thumm, and Renate Voss for excellent technical assistance.

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Lohwasser, U., Rehman Arif, M.A. & Börner, A. Discovery of loci determining pre-harvest sprouting and dormancy in wheat and barley applying segregation and association mapping. Biol Plant 57, 663–674 (2013). https://doi.org/10.1007/s10535-013-0332-2

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  • DOI: https://doi.org/10.1007/s10535-013-0332-2

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