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Exogenous application of trehalose induced H2O2 production and stomatal closure in Vicia faba

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

Trehalose can reduce stomatal aperture by a hydrogen-peroxide-dependent pathway in Vicia faba L. (cv. Daqingpi) resulting in significantly lower values of net photosynthetic rate (PN), stomatal conductance (gs), and transpiration rate (E). At 8 and 24 h, the lower PN in trehalose-treated plants was accompanied by significant decrease in intercellular CO2 concentration (ci) suggesting that the reduction of PN was caused by stomatal limitation. At 48 and 72 h, trehalose decreased apparent carboxylation efficiency (PN/ci) and did not decrease ci and gs compared with controls; therefore the reduction in photosynthesis was caused by non-stomatal limitation. Trehalose treatment resulted in significantly higher effective photochemical efficiency of PS II (ΦPSII) and did not affect maximum photochemical efficiency of PS II (Fv/Fm). At 24, 48, and 72 h, trehalose decreased non-photochemical quenching (NPQ) and increased photochemical quenching (qP). Our results suggest that trehalose did not damage photosynthetic reaction centers.

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Abbreviations

ABA:

abscisic acid

c a :

ambient CO2 concentration

ci :

intercellular CO2 concentration

E:

transpiration rate

Fv/Fm :

variable to maximum fluorescence ratio (maximum photochemical efficiency of photosystem II)

gs :

stomatal conductance

NPQ:

non-photochemical quenching

PAR:

photosynthetically active radiation

PN :

net photosynthetic rate

PN/ci :

apparent carboxylation efficiency

qP:

photochemical quenching

ROS:

reactive oxygen species

TPP:

trehalose phosphate phosphatase

TPS:

trehalose phosphate synthase

ΦPS2 :

effective photochemical efficiency of PS II

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, 310058, Hangzhou, P.R. China

    J. Gao, N. Wang, S. -S. Xu, Y. Li, Y. Wang & G. -X. Wang

Authors
  1. J. Gao
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  2. N. Wang
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  3. S. -S. Xu
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  4. Y. Li
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  5. Y. Wang
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  6. G. -X. Wang
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Corresponding author

Correspondence to G. -X. Wang.

Additional information

Acknowledgements: This work was financed by National High Technology Research and Development Program of China (2011AA100503). The first two authors contributed equally to this study.

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Gao, J., Wang, N., Xu, S.S. et al. Exogenous application of trehalose induced H2O2 production and stomatal closure in Vicia faba . Biol Plant 57, 380–384 (2013). https://doi.org/10.1007/s10535-012-0285-x

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  • DOI: https://doi.org/10.1007/s10535-012-0285-x

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