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Water deficit and recovery response of Medicago truncatula plants expressing the ELIP-like DSP22

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

In this article, we present the response of Medicago truncatula Gaert. cv. Jemalong plants expressing constitutively the Dsp22 gene from Craterostigma plantagineum to water stress and rehydration. The Dsp22 gene encodes an ELIP-like protein thought to protect the chloroplast against photooxidative damage during the dehydration and rehydration. The Dsp22 transgenic homozygous M. truncatula plants showed higher amount of chlorophyll (Chl), lower Chl a/Chl b ratio and higher actual efficiency of energy conversion in photosystem 2 (ΦPSII) after rehydration, when compared to the wild type. The combined data from the Chl a fluorescence analysis, pigment quantification and biomass accumulation showed that transgenic M. truncatula plants are able to recover from water deprivation better than wild type plants.

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Abbreviations

Chl:

chlorophyll

DSP22:

desiccation stress protein (22 kDa)

ELIPs:

early light induced proteins

Fv/Fm :

variable to maximum Chl fluorescence ratio (maximum quantum efficiency of photosystem 2)

LA:

leaf blade area

LHC 2:

light harvesting complexes of the photosystem 2

PAR:

photosynthetically active radiation

PSII:

photosystem II

qN:

non-photochemical quenching

qP:

photochemical quenching

RWC:

relative water content

SWC:

soil water content

WD:

water deficit

WDR:

water deficit recovery

WW:

well watered

ΦPSII :

actual efficiency of energy conversion in photosystem 2

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Authors and Affiliations

  1. Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2781-901, Oeiras, Portugal

    S. S. Araújo, A. S. Duque & P. Fevereiro

  2. Faculdade de Medicina Veterinária, Instituto de Investigação Científica e Tropical, 1300-477, Lisboa, Portugal

    S. S. Araújo & D. Santos

  3. Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, 1749-016, Lisboa, Portugal

    J. M. Silva, A. B. Silva & P. Fevereiro

  4. BioFIG, Faculdade de Ciências da Universidade de Lisboa, 1749-016, Lisboa, Portugal

    J. M. Silva & A. B. Silva

Authors
  1. S. S. Araújo
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  2. A. S. Duque
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  3. J. M. Silva
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  4. D. Santos
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  5. A. B. Silva
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  6. P. Fevereiro
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Corresponding author

Correspondence to S. S. Araújo.

Additional information

Acknowledgements: We thank Prof. D. Bartels (IMBIO, Germany) for kindly providing the Dsp22 cDNA clone from Craterostigma plantagineum, Dr. V. Gemas (ITQB, Portugal) for support in statistical analysis and Dr A.M. De Almeida (IICT, Portugal) for critically reviewing the manuscript. This research was supported by Fundação para a Ciência e Tecnologia (Lisbon, Portugal), in the form of fellowships (S.S. Araújo BD/5225/2001 and Ciência 2008 Research Contract; A.S. Duque BPD/74784/2010, and D. Santos BPD/21968/2005 and Ciência 2007 Research Contract) and research project POCTI/BIO/56659/2004.

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Araújo, S.S., Duque, A.S., Silva, J.M. et al. Water deficit and recovery response of Medicago truncatula plants expressing the ELIP-like DSP22. Biol Plant 57, 159–163 (2013). https://doi.org/10.1007/s10535-012-0235-7

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  • DOI: https://doi.org/10.1007/s10535-012-0235-7

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