Abstract
Bacillus cereus C1L was demonstrated to induce systemic resistance in Lilium formosanum against leaf blight caused by Botrytis elliptica. Suppression subtractive hybridization library of L. formosanum triggered by B. cereus C1L were screened and 3 differentially expressed genes were identified. Based on sequence analysis, these genes encoding putative glycine-rich protein, metallothionein-like protein, and PsbR protein of photosystem 2, were designated LfGRP1, LfMT1, and LfPsbR, respectively. The results of Northern blot analysis showed that expressions of LfGRP1, LfMT1 and LfPsbR increased in response to B. elliptica infection. On the other hand, expression of LfMT1 increased but expressions of LfGRP1 and LfPsbR decreased when the rhizosphere of L. formosanum was drenched with suspension of B. cereus C1L with or without subsequent challenge with B. elliptica on lily leaves. Similar expression profiles of homologues of LfGRP1, LfMT1, and LfPsbR (named LsGRP1, LsMT1, and LsPsbR, respectively) were presented in Lilium oriental hybrid Star Gazer. In addition, application of the photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, on the leaves reduced disease severity and expressions of LsGRP1 and LsPsbR just as that in response to B. cereus C1L treatment.
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Abbreviations
- SSH:
-
suppression subtractive hybridization
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
References
Akashi, K., Nishimura, N., Ishida, Y., Yokota, A.: Potent hydroxyl radical-scavenging activity of drought-induced type-2 metallothionein in wild watermelon. — Biochem. biophys. Res. Commun. 323: 72–78, 2004.
Allahverdiyeva, Y., Mamedov, F., Suorsa, M., Styring, S., Vass, I., Aro, E.-M.: Insights into the function of PsbR protein in Arabidopsis thaliana. — Biochem. biophys. Acta 1767: 677–685, 2007.
Bakker, P.A.H.M., Pieterse, C.M.J., van Loon, L.C.: Induced systemic resistance by fluorescent Pseudomonas spp. — Phytopathology 97: 239–243, 2007.
Brooks, A.V.: Lily Diseases and Disorders. — Universe Books, New York 1980.
Buchanan-Wollaston, V.: Isolation of cDNA clones for genes that are expressed during leaf senescence in Brassica napus. — Plant Physiol. 105: 839–846, 1994.
Cartieaux, F., Thibaud, M.C., Zimmerli, L., Lessard, P., Sarrobert, C., David, P., Gerbaud, A., Robaglia, C., Somerville, S., Nussaume, L.: Transcriptome analysis of Arabidopsis colonized by a plant-growth promoting rhizobacterium reveals a general effect on disease resistance. — Plant J. 36: 177–188, 2003.
Chen, C.Y., Huang, H.E.: Salicylic acid-induced resistance of lily leaves against Botrytis elliptica. — Plant Pathol. Bull. 6: 76–82, 1997.
Chen, J., Jacobson, L.M., Handelsman, J., Goodman, R.M.: Compatibility of systemic acquired resistance and microbial biocontrol for suppression of plant disease in a laboratory assay. — Mol. Ecol. 5: 73–80, 1996.
Chiou, A.L., Wu, W.S.: Isolation, identification and evaluation of bacterial antagonists against Botrytis elliptica on lily. — J. Phytopathol. 149: 319–324, 2001.
Chiou, A.L., Wu, W.S.: Formulation of Bacillus amyloliquefaciens B190 for control of lily grey mould (Botrytis elliptica). — J. Phytopathol. 151: 13–18, 2003.
Choi, D., Kim, H.M., Yun, H.K., Park, J.A., Kim, W.T., Bok, S.H.: Molecular cloning of a metallothionein-like gene from Nicotiana glutinosa L. and its induction by wounding and tobacco mosaic virus infection. — Plant Physiol. 112: 353–359, 1996.
Coupe, S.A., Taylor, J.E., Roberts, J.A.: Characterization of an mRNA encoding a metallothionein-like protein that accumulates during ethylene-promoted abscission of Sambucus nigra L. leaflets. — Planta 197: 442–447, 1995.
De Las Rivas, J., Heredia, P., Roman, A.: Oxygen-evolving extrinsic proteins (PsbO, P, Q, R): bioinformatic and functional analysis. — Biochem. biophys. Acta 1767: 575–582, 2007.
Durrant, W.E., Dong, X.: Systemic acquired resistance. — Annu. Rev. Phytopathol. 42: 185–209, 2004.
Etscheid, M., Kümper, S., Riesner, D.: Accumulation of a metallothionein-like mRNA in Norway spruce under environmental stress. — J. Phytopathol. 147: 207–213, 1999.
Hamer, D.H.: Metallothionein. — Annu. Rev. Biochem. 55: 913–951, 1986.
Hsieh, H.M., Liu, W.K., Huang, P.C.: A novel stress-inducible metallothionein-like gene from rice. — Plant mol. Biol. 28: 381–389, 1995.
Huang, C.J., Wang, T.K., Chung, S.C., Chen, C.Y.: Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28–9. — J. Biochem. mol. Biol. 38: 82–88, 2005.
Kessmann, H., Staub, T., Hofmann, C., Maetzke, T., Herzog, J., Ward, E., Uknes, S., Ryals, J.: Induction of systemic acquired disease resistance in plants by chemicals. — Annu. Rev. Phytopathol. 32: 439–459, 1994.
Kim, S., Ahn, I.P., Lee, Y.H.: Analysis of genes expressed during rice-Magnaporthe grisea interactions. — Mol. Plant-Microbe Interact. 14: 1340–1346, 2001.
Kloepper, J.W., Ryu, C.-M., Zhang, S.: Induced systemic resistance and promotion of plant growth by Bacillus spp. — Phytopathology 94: 1259–1266, 2004.
Liu, Y.H., Huang, C.J., Chen, C.Y.: Evidence of induced systemic resistance against Botrytis elliptica in lily. — Phytopathology 98: 830–836, 2008.
Liu, P., Goh, C.J., Loh, C.S., Pua, E.C.: Differential expression and characterization of three metallothionein-like genes in Cavendish banana (Musa acuminata). — Physiol. Plant. 114: 241–250, 2002.
Lu, Y.Y., Chen, C.Y.: Probenazole-induced resistance of lily leaves against Botrytis elliptica. — Plant Pathol. Bull. 7: 134–140, 1998.
Lu, Y.Y., Chen, C.Y.: Molecular analysis of lily leaves in response to salicylic acid effective towards protection against Botrytis elliptica. — Plant Sci. 169: 1–9, 2005.
Lu, Y.Y., Liu, Y.H., Chen, C.Y.: Stomatal closure, callose deposition and increase of LsGRP1-corresponding transcript in probenazole-induced resistance against Botrytis elliptica in lily. — Plant Sci. 172: 913–919, 2007.
MacDaniels, L.H., Horst, R.K.: Lily Disease Problems in North America. — Universe Books, New York 1980.
Mahmood, T., Jan, S., Komatsu, S.: Proteomic analysis of bacterial blight defence signalling pathway using transgenic rice overexpressing thaumatin-like protein. — Biol. Plant. 53: 285–293, 2009.
Reid, S.J., Ross, G.S.: Up-regulation of two cDNA clones encoding methallothionein-like proteins in apple fruit during cool storage. — Physiol. Plant. 100: 183–189, 1997.
Robinson, N.J., Tommey, A.M., Kuske, C., Jackson, P.J.: Plant metallothioneins. — Biochem. J. 295: 1–10, 1993.
Suorsa, M., Sirpiö, S., Allahverdiyeva, Y., Paakkarinen, V., Mamedov, F., Styring, S., Aro, E. M.: PsbR, a missing link in the assembly of the oxygen-evolving complex of plant photosystem II. — J. biol. Chem. 281: 145–150, 2006.
Van Baarlen, P., Staats, M., Van Kan, J.A.L.: Induction of programmed cell death in lily by the fungal pathogen Botrytis elliptica. — Mol. Plant. Pathol. 5: 559–574, 2004.
Van Loon, L.C., Bakker, P.A.H.M., Pieterse, C.M.J.: Systemic resistance induced by rhizosphere bacteria. — Annu. Rev. Phytopathol. 36: 453–483, 1998.
Zehnder, G.W., Murphy, J.F., Sikora, E.J., Kloepper, J.W.: Application of rhizobacteria for induced resistance. — Eur. J. Plant Pathol. 107: 39–50, 2001.
Zhou, J., Goldsbrough, P.B.: Structure, organization and expression of the metallothionein gene family in Arabidopsis. — Mol. gen. Genet. 248: 318–328, 1995.
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This study was financially supported by National Science Council, Taiwan, Republic of China. The two first authors contributed equally to the present work.
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Liu, Y.H., Huang, C.J. & Chen, C.Y. Identification and transcriptional analysis of genes involved in Bacillus cereus-induced systemic resistance in Lilium . Biol Plant 54, 697–702 (2010). https://doi.org/10.1007/s10535-010-0123-y
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DOI: https://doi.org/10.1007/s10535-010-0123-y