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Direct somatic embryogenesis and plant regeneration from immature explants of chickpea

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Biologia Plantarum

Abstract

A protocol for plant regeneration via somatic embryogenesis was developed in two chickpea (Cicer arietinum L.) cultivars ICCV-10 and Annigeri. Somatic embryos were induced from immature cotyledons on Murashige and Skoog’s (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), α-naphthaleneacetic acid (NAA) and picloram alone or in combination with 0.5 — 2.0 mg dm−3 N6-benzylaminopurine (BA) or kinetin (KIN). NAA was better for somatic embryo induction compared to other auxins. The well formed, cotyledonary shaped embryos germinated into plantlets with 36.6 % frequency on MS medium supplemented with 2.0 mg dm−3 BA + 0.5 mg dm−3 abscisic acid (ABA). The frequency of embryogenesis and plantlet regeneration was higher in cv. ICCV-10 as compared to cv. Annigeri. Regenerated plants were transferred to soil (40 % survival) and grown to maturity. Histological studies of explants at various developmental stages of somatic embryogenesis reveled that somatic embryos developed directly from the cotyledon cells and they were single cell origin.

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Abbreviations

ABA:

abscisic acid

BA:

N6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acids

KIN:

kinetin

MS:

Murashige and Skoog

NAA:

α-naphthaleneacetic acid

2,4,5-T:

2,4,5-trichlorophenoxyacetic acids

TDZ:

thidiazuron

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Acknowledgements

Authors are grateful to Prof. Udayakumar, Department of Crop Physiology, University of Agricultural Science, GKVK, Bangalore for help in critically analyzing and preparation of this manuscript.

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Authors and Affiliations

  1. Department of Botany, Gulbarga University, Gulbarga, 585106, Karnataka, India

    S. Kiran Ghanti, K. G. Sujata & M. Srinath Rao

  2. Department of Genetics, Osmania University, Hyderabad, 500007, India

    P. B. Kavi Kishor

  3. Department of Crop physiology, University of Agricultural Science, GKVK, Karnataka, Bangalore, 560065, India

    S. Kiran Ghanti

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  1. S. Kiran Ghanti
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  2. K. G. Sujata
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  3. M. Srinath Rao
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  4. P. B. Kavi Kishor
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Correspondence to S. Kiran Ghanti.

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Kiran Ghanti, S., Sujata, K.G., Srinath Rao, M. et al. Direct somatic embryogenesis and plant regeneration from immature explants of chickpea. Biol Plant 54, 121–125 (2010). https://doi.org/10.1007/s10535-010-0018-y

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  • DOI: https://doi.org/10.1007/s10535-010-0018-y

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