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Somatic embryogenesis and organogenesis inGuizotia Abyssinica

  • Developmental Biology/Morphogenesis
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Summary

Induction of somatic embryogenesis, shoot organogenesis, and subsequent plant regeneration in niger seem to be dependent on genotype, choice of explant, and composition of media growth regulators. Two distinct regeneration protocols have been developed for somatic embryogenesis and shoot organogenesis. Somatic embryogenesis was induced from epicotyls and cotyledonary explants (9 to 35%) (but not from hypocotyls and roots) in presence of 2,4-dichlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid, and 2,4,5-trichlorophenoxypropionic acid. These embryos matured in MS medium containing Kinetin plus naphthalene acetic acid (NAA), Kinetin plus Zeatin, and Kinetin plus abscisic acid (ABA). Matured embryos could be germinated on LS and MS basal media without hormones. Non-embryogenic callus initiated on Linsmaier and Skoog’s (LS) medium from cotyledons of six different genotypes produced shoots (9 to 32%) on Murashige and Skoog’s (MS) medium fortified with 6-benzylaminopurine (BAP, 0.5 mg · liter−1), and BAP (1 mg · liter−1) plus NAA (0.1 mg · liter−1). These shoots were rooted with 100% frequency by using indole-3-acetic acid or NAA and transferred successfully to the soil.

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Authors and Affiliations

  1. Department of Genetics, Osmania University, 500 007, Hyderabad, India

    T. P. Reddy & P. B. Kavi Kishor

  2. Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, 19104, Philadelphia, Pennsylvania

    Sarvesh Adda

Authors
  1. Sarvesh Adda
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  2. T. P. Reddy
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  3. P. B. Kavi Kishor
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Adda, S., Reddy, T.P. & Kavi Kishor, P.B. Somatic embryogenesis and organogenesis inGuizotia Abyssinica . In Vitro Cell Dev Biol - Plant 30, 104–107 (1994). https://doi.org/10.1007/BF02632137

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  • DOI: https://doi.org/10.1007/BF02632137

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