Abstract
An efficient regeneration system for Phaseolus vulgaris was developed from mature seeds germinated on Murashige and Skoog (MS) medium supplemented with thidiazuron or N6-benzylaminopurine (BA) for 6 d. Using cotyledonary nodes, multiple buds were induced on the MS medium supplemented with 5.0 mg dm−3 BA with the induction frequency 71.9 % after 4-week culture. The buds were then transferred onto shoot formation medium containing 1.0 mg dm−3 BA, 0.1 mg dm−3 gibberellic acid and 2.0 mg dm−3 silver nitrate. The addition of AgNO3 enhanced the frequency of the shoot formation from 61.3 to 87.6 %. Root induction medium was half-strength MS medium with 0.75 mg dm−3 indolebutyric acid and 0.02 mg dm−3 BA. The average root frequency was 84.3 %. The regenerated plantlets with healthy roots grew successfully when transferred to soil. Using this system we obtained over 10 regenerated plantlets from one explant.
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Abbreviations
- BA:
-
N6-benzylaminopurine
- GA3 :
-
gibberellic acid
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige and Skoog
- TDZ:
-
thidiazuron
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Dang, W., Wei, Z.M. High frequency plant regeneration from the cotyledonary node of common bean. Biol Plant 53, 312–316 (2009). https://doi.org/10.1007/s10535-009-0056-5
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DOI: https://doi.org/10.1007/s10535-009-0056-5