Abstract
Adventitious shoot regeneration from root, hypocotyl, cotyledon and primary leaf explants of safflower (Carthamus tinctorius L.) was studied. Shoot regeneration was promoted by benzyladenine (BA) + naphthaleneacetic acid (NAA), BA + indole-3-butyric acid (IBA), kinetin + NAA and thidiazuron (TDZ) + NAA incorporated in Murashige and Skoog (MS) basal medium. High frequency of shoot regeneration and high number of shoots per regenerating explant were obtained on a wide range of TDZ + NAA combinations. Proliferated shoots were elongated in MS + 0.5 mg dm−3 kinetin and well-developed shoots were rooted in half strength MS + 0.5 mg dm−3 NAA. Rooted shoots were successfully acclimatized and established in soil.
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Abbreviations
- BA:
-
N6-benzyladenine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige and Skoog (1962) medium
- NAA:
-
α-naphthaleneacetic acid
- TDZ:
-
1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (thidiazuron)
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Radhika, K., Sujatha, M. & Nageshwar Rao, T. Thidiazuron stimulates adventitious shoot regeneration in different safflower explants. Biol Plant 50, 174–179 (2006). https://doi.org/10.1007/s10535-006-0003-7
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DOI: https://doi.org/10.1007/s10535-006-0003-7