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Effects of 28-homobrassinolide on nickel uptake, protein content and antioxidative defence system in Brassica juncea

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Biologia Plantarum

Abstract

The effects of 28-homobrassinolide (HBL) on nickel uptake, protein content and activities of antioxidative enzymes were determined in the seedlings of Brassica juncea L. The seeds were treated with different concentrations (0, 0.01, 1 and 100 nM) of HBL for 8 h and then sown in the Petri plates containing various concentrations (0, 25, 50 and 100 mg dm−3) of nickel. After 7 d, observations were made on shoot and root length, Ni uptake, protein content and activities of antioxidative enzymes (guaiacol peroxidase, catalase, glutathione reductase, ascorbate peroxidase and superoxide dismutase). The growth of seedlings was inhibited by Ni, however, less after HBL pre-treatment. The protein content and antioxidative enzyme activities were also increased by HBL treatment.

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Abbreviations

APX:

ascorbate peroxidase

BCF:

bioconcentration factor

BRs:

brassinosteroids

CAT:

catalase

GR:

glutathione reductase

HBL:

28-homobrassinolide

POD:

guaiacol peroxidase

ROS:

reactive oxygen species

SOD:

superoxide dismutase

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Correspondence to R. Bhardwaj.

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Sharma, P., Bhardwaj, R., Arora, N. et al. Effects of 28-homobrassinolide on nickel uptake, protein content and antioxidative defence system in Brassica juncea . Biol Plant 52, 767–770 (2008). https://doi.org/10.1007/s10535-008-0149-6

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  • DOI: https://doi.org/10.1007/s10535-008-0149-6

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