Abstract
The effects of 28-homobrassinolide (HBL) on nickel uptake, protein content and activities of antioxidative enzymes were determined in the seedlings of Brassica juncea L. The seeds were treated with different concentrations (0, 0.01, 1 and 100 nM) of HBL for 8 h and then sown in the Petri plates containing various concentrations (0, 25, 50 and 100 mg dm−3) of nickel. After 7 d, observations were made on shoot and root length, Ni uptake, protein content and activities of antioxidative enzymes (guaiacol peroxidase, catalase, glutathione reductase, ascorbate peroxidase and superoxide dismutase). The growth of seedlings was inhibited by Ni, however, less after HBL pre-treatment. The protein content and antioxidative enzyme activities were also increased by HBL treatment.
Abbreviations
- APX:
-
ascorbate peroxidase
- BCF:
-
bioconcentration factor
- BRs:
-
brassinosteroids
- CAT:
-
catalase
- GR:
-
glutathione reductase
- HBL:
-
28-homobrassinolide
- POD:
-
guaiacol peroxidase
- ROS:
-
reactive oxygen species
- SOD:
-
superoxide dismutase
References
Aebi, H.: Catalase.-In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Pp. 673–684. Verlag-Chemie, Weinham 1974.
Ali, B., Hayat, S., Ahmad, A.: Response of germinating seeds of Cicer arietinum to 28-homobrassinolide and/or potassium.-J. gen. appl. Plant Physiol. 31: 55–63, 2005.
Allen, S.E., Grimshaw, H.M., Parkinson, J.A., Quarmby, C., Roberts, J.D.: Chemical Analysis.-In: Chapmann, S.B. (ed.): Methods in Plant Ecology. Pp. 424–426. Blackwell Scientific Publications, Oxford-London 1976.
Bailey, N.T.J.: Statistical Methods in Biology (3rd Ed).-The English University Press, London 1995.
Bajguz, A.: Blockage of heavy metal accumulation in Chlorella vulgaris cells by 24-epibrassinolide.-Plant Physiol. Biochem. 38: 797–801, 2000a.
Bajguz, A.: Effects of brassinosteroids on nucleic acids and protein in cultured cells in Chlorella vulgaris.-Plant Physiol. Biochem. 38: 209–215, 2000b.
Bhardwaj, R., Arora, H.K., Nagar, P.K., Thukral, A.K.: Brassinosteroids — a novel group of plant hormones.-In: Trivedi, P.C. (ed.): Plant Molecular Physiology — Current Scenario and Future Projections. Pp. 58–84. Aaviskar Publisher, Jaipur 2006.
Bilkisu, A.A., Xiao-Gang, G., Qing-Lei, G., Yong-Hua, Y.: Brassinolide amelioration of aluminium toxicity in mungbean seedling growth.-J. Plant Nutr. 26: 1725–1734, 2003.
Cao, X., Ma, L.Q., Tu, C.: Antioxidative responses to arsenic in the arsenic-hyperaccumulator Chinese brake fern (Pteris vittata L.).-Environ. Pollut. 128: 317–325, 2004.
Carlberg, I., Mannervik, B.: Purification of the flavoenzyme glutathione reductase from rat liver.-J. biol. Chem. 250: 5475–5480, 1975.
Clouse, S.D., Sasse, J.M.: Brassinosteroids: essential regulators of plant growth and development.-Annu. Rev. Plant Physiol. Plant mol. Biol. 49: 427–451, 1998.
Dhaubhadel, S., Browning, K.S., Gallie, D.R., Krishna, P.: Brassinosteroid functions to protect the translational machinery and heat-shock protein synthesis following thermal stress.-Plant J. 29: 681–691, 2002.
Dhaubhadel, S., Chaudhary, S., Dobinson, K.F., Krishna, P.: Treatment with 24-epibrassinolide (a brassinosteroid) increases the basic thermotolerance of Brassica napus tomato seedlings.-Plant mol. Biol. 40: 333–342, 1999.
El-Shintinawy, F., El-Ansari, A.: Differential effect of Cd2+ and Ni2+ on amino acid metabolism in soybean seedling.-Biol. Plant. 43: 74–84, 2000.
Fariduddin, Q., Ahmad, A., Hayat, S.: Response of Vigna radiata to foliar application of 28-homobrassinolide and kinetin.-Biol. Plant. 48: 465–468, 2004.
Hayat, S., Ahmad, A.: Soaking seeds of Lens culinaris with 28-homobrassinolide increased nitrate reductase activity and grain yield in the field in India.-Ann. appl. Biol. 143: 121–124, 2003.
Janeczko, A., Koscielniak, J., Pilipowicz, M., Szarek-Lukaszewsa, G., Skoczowspi, A.: Protection of winter rape photosystem 2 by 24-epibrassinolide under cadmium stress.-Photosynthetica 43: 293–298, 2005.
Janeczko, A. Gullner, G., Skoczowski, A., Dubert, F., Barna, B.: Effects of brassinosteroid infiltration prior to cold treatment on ion leakage and pigment contents in rape leaves.-Biol. Plant. 51: 355–358, 2007.
Kalinich, J.F., Mandava, N.B., Todhunter, J.A.: Relationship of nucleic acid metabolism on brassinolide-induced responses in beans.-Plant Physiol. 120: 207–214, 1985.
Kaur, S., Bhardwaj, R.: Brassinosteroids regulated heavy metals uptake in Brassica campestris L.-In: Annual Meeting of the American Society of Plant Biologists “Plant Biology 2003”. P. 628. Honolulu 2003.
Kono, Y.: Generation of superoxide radical during autooxidation of hydroxylamine and an assay for superoxide dismutase.-Arch. Biochem. Biophys. 186: 189–195, 1978.
Kovtun, Y., Chiu, W.L., Tena Sheen, J.: Functional analysis of oxidative stress — activated mitogen — activated protein kinase cascade in plants.-Proc. nat. Acad. Sci. USA 97: 2940–2945, 2000.
Lin, Y.C., Kao, C.H.: Proline accumulation induced by excess nickel in detached rice leaves.-Biol. Plant. 51: 351–354, 2007.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.L.: Protein measurement with Folin phenol reagent.-J. biol. Chem. 193: 265–275, 1951.
Maksimović, I., Kastori, R., Krstić, L., Luković, J.: Steady presence of cadmium and nickel affects root anatomy, accumulation and distribution of essential ions in maize seedlings.-Biol. Plant. 51: 589–592, 2007.
Mandava, N.B.: Plant growth promoting brassinosteroids.-Annu. Rev. Plant Physiol. Plant mol. Biol. 39: 23–52, 1988.
Mazorra, L.M., Nunez, M., Hechavarria, M., Coll, F., Sanchez-Blanco, M.J.: Influence of brassinosteroids on antioxidants enzymes activity in tomato under different temperature.-Biol. Plant. 45: 593–596, 2002.
Meharg, A.A.: The role of the plasmalemma in metal tolerance in angiosperms.-Physiol. Plant. 88: 191–198, 1993.
Metwally, A., Finkemeier, I., Georgi, M., Dietz, K.J.: Salicylic acid alleviates the cadmium toxicity in barley seedlings.-Plant Physiol. 132: 272–281, 2003.
Nakano, Y., Asada, A.: Hydrogen peroxide is scavenged by ascorbate specific-peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867–880, 1981.
Nunez, M., Mazzafera, P., Mazorra, L.M., Siqueira, W.J., Zullo, M.A.T.: Influence of brassinosteroid analogue on antioxidant enzymes in rice grown in culture medium with NaCl.-Biol. Plant. 47: 67–70, 2003.
Ozdemir, F., Bor, M., Demiral, T., Turkan, I.: Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and antioxidative system of rice (Oryza sativa L.) under salinity stress.-Plant Growth Regul. 42: 203–211, 2004.
Panda, S.K., Chaudhury, I., Khan, M.H.: Heavy metals induced lipid peroxidation and affect antioxidants in wheat leaves.-Biol. Plant. 46: 289–294, 2003.
Putter, J.: Peroxidase.-In: Bergmeyer, H.U. (ed): Methods of Enzymatic Analysis. Pp. 685–690. Verlag-Chemie, Weinham 1974.
Sharma, P., Bhardwaj, R.: Effect of 24-epibrassinolide on growth and metal uptake in Brassica juncea L. under copper metal stress.-Acta Physiol. Plant. 29: 259–263, 2007a.
Sharma, P., Bhardwaj, R.: Effect of 24-epibrassinolide on seed germination, seedling growth and heavy metals uptake in Brassica juncea L.-J. gen. appl. Plant. Physiol. 33: 59–73, 2007b.
Vahala, J., Keinanen, M., Schutzendubel, A., Polle, A., Kangasjarvi, J.: Differential effects of elevated ozone on two hybrid aspen genotypes predisposed to chronic ozone fumigation role of ethylene and salicylic acid.-Plant Physiol. 132: 196–205, 2003.
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Sharma, P., Bhardwaj, R., Arora, N. et al. Effects of 28-homobrassinolide on nickel uptake, protein content and antioxidative defence system in Brassica juncea . Biol Plant 52, 767–770 (2008). https://doi.org/10.1007/s10535-008-0149-6
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DOI: https://doi.org/10.1007/s10535-008-0149-6