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In vitro plant regeneration from leaf explants of Ophiorrhiza japonica

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  • Published:
Biologia Plantarum

Abstract

An efficient in vitro plant regeneration system from leaves of Ophiorrhiza japonica Blume was established for the first time. Callus formation rate was more than 90.4 % from leaf segments on Murashige and Skoog (MS) supplemented with either α-naphthaleneacetic acid (NAA) alone or in combination with 6-benzyladenine (BA). The highest shoot regeneration (78.9 %) was achieved on MS medium containing 2.0 mg dm−3 BA and 0.2 mg dm−3 NAA, with an average of 9.4 shoots developed per leaf segment. Shoot regeneration was also improved when the leaf explants were cultured in MS basal medium supplemented with 0.5 % (m/v) polyvinylpyrrolidone (PVP). The leaf explants from seedlings with age of about 18–27 d showed the highest shoot regeneration. The regenerated shoots were rooted on half-strength basal MS medium supplemented with 0.5 mg dm−3 indole-3-butyric acid (IBA), which averagely produced 24.8 roots per shoot. The plantlets were transferred to soil, where 100 % survived after 1 month of acclimatization.

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Abbreviations

BA:

6-benzyladenine

CPT:

camptothecin

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog

NAA:

naphthalene acetic acid

PVP:

polyvinylpyrrolidone

WPM:

woody plant medium

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Authors and Affiliations

  1. Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, Shanghai, 200234, P.R. China

    G.-Y. Kai, L.-M. Dai, X.-Y. Mei, W. Wang, Y. Lu, Z.-Y. Qian & G.-Y. Zhou

  2. Laboratory of Quality Biotechnology, School of Life Sciences, Fujian Agriculture and Forest University, Fuzhou, 350002, P.R. China

    J.-G. Zheng

Authors
  1. G.-Y. Kai
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  2. L.-M. Dai
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  3. X.-Y. Mei
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  4. J.-G. Zheng
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  5. W. Wang
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  6. Y. Lu
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  7. Z.-Y. Qian
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  8. G.-Y. Zhou
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Correspondence to G.-Y. Kai.

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Kai, GY., Dai, LM., Mei, XY. et al. In vitro plant regeneration from leaf explants of Ophiorrhiza japonica . Biol Plant 52, 557–560 (2008). https://doi.org/10.1007/s10535-008-0110-8

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  • DOI: https://doi.org/10.1007/s10535-008-0110-8

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