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Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis

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Biologia Plantarum

Abstract

Leaf explants of Phalaenopsis amabilis var. formosa formed clusters of somatic embryos directly from epidermal cells without an intervening callus within 20 – 30 d when cultured on 1/2-strength modified Murashige and Skoog medium supplemented with 0.1, 1 and 3 mg dm−3 TDZ. Repetitive production of embryos involved secondary embryogenesis could be obtained by culturing segments of embryogenic masses on TDZ-containing media. Plantlet conversion from embryos was successfully achieved on regulator-free growth medium.

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Abbreviations

MS:

Murashige and Skoog (1962) medium

NAA:

naphthaleneacetic acid

TDZ:

thidiazuron (1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea

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Authors and Affiliations

  1. Institute of Biotechnology, National University of Kaohsiung, Kaohsiung, 811, Taiwan, Republic of China

    J. T. Chen

  2. Department of Life Sciences, National University of Kaohsiung, Kaohsiung, 811, Taiwan, Republic of China

    J. T. Chen

  3. Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 115, Taiwan, Republic of China

    W. C. Chang

Authors
  1. J. T. Chen
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  2. W. C. Chang
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Correspondence to J. T. Chen.

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Chen, J.T., Chang, W.C. Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis . Biol Plant 50, 169–173 (2006). https://doi.org/10.1007/s10535-006-0002-8

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  • DOI: https://doi.org/10.1007/s10535-006-0002-8

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