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Direct somatic embryogenesis from leaves, cotyledons and hypocotyls of Hippophae rhamnoides

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Biologia Plantarum

Abstract

Plant regeneration via direct somatic embryogenesis from cotyledons, hypocotyls and leaves in seabuckthorn (Hippophae rhamnoides L.) was achieved. The influences of basal media, carbon sources, plant growth regulators (PGRs) with different concentrations and combinations on embryogenesis capacity of explants were studied. The highest frequency of somatic embryos production and germination was obtained on Schenk and Hildebrandt medium (SH) supplemented with 1.0 mg dm−3 kinetin and 0.2, 0.5 mg dm−3 indole-3-acetic acid. Granulated sugar was the optimal carbon source. The embryo-derived plantlets with well-developed roots and shoots were transferred successfully to the greenhouse with a maximum survival rate of 55 %. Histological observation revealed that the somatic embryos were similar to those of zygotic embryos.

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Abbreviations

BA:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

FAA:

formalin acetic acid ethanol

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

KIN:

kinetin

MS medium:

Murashige and Skoog medium

NAA:

α-naphthaleneacetic acid

NN69 medium:

Nitsch and Nitsch medium

PGR:

plant growth regulator

SH medium:

Schenk and Hildebrandt medium

WPM medium:

Lloyd and McCown medium

References

  • Abdoulaye, T. (ed.): Somatic Embryogenesis, Embryo Conversion, Micropropagation and Factors Affecting Genetic Transformation of Theobroma cacao L.-UMI Dissertation Services, Pennsylvania 2000.

  • Akhtar, N., Kumari, N., Pandey, S., Ara, H., Singh, M., Jaiswal, U., Jaiwal, V.S., Jain, S.M.: Somatic embryogenesis in tropical fruit trees.-In: Jain, S.M., Gupta, P.K., Newton, R.J. (ed.): Somatic Embryogenesis in Woody Plants. Vol. 6. Pp. 93–140. Kluwer Academic Publishers, Dordrecht 2000.

    Google Scholar 

  • Daigny, G., Paual, H., Sangwan, R.S., Sangwan-Norreel, B.S.: Factors influencing secondary somatic embryogenesis in Malus × domestica Borkh. (cv. ‘Gloster 69’).-Plant Cell Rep. 16: 153–157, 1996.

    CAS  Google Scholar 

  • Ganesan, M., Jayabalan, N.: Evaluation of haemoglobin (erythrogen): for improved somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L. cv. SVPR2).-Plant Cell Rep. 23: 181–187, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Hassanein, A.M.: Somatic embryogenesis of cucumber (Cucumis sativus L.) using seed cuttings obtained from premature fruit.-Plant Biotechnol. 20: 275–281, 2003.

    CAS  Google Scholar 

  • Knyazev, A.V., Chemeris, A.V., Vakhitov, V.A.: [Morphogenesis of Hippophae rhamnoides L. in vitro.]-Rast. Resurs. 39: 107–115, 2003. [In Russ.]

    Google Scholar 

  • Ladyman, J.A.R., Girard, B.: Cucumber somatic development on various gelling agents and carbohydrates sources.-Hort. Sci. 27: 164–165, 1992.

    Google Scholar 

  • Li, H.Q., Liang, C.Y., Huang, Y.W., Guo, J.Y.: [Factors influencing the developments of secondary embryoids and plant regeneration on cassava (Manihot esculenta Crantz).]-Guihaia 19: 246–250, 1999. [In Chin.]

    CAS  Google Scholar 

  • Litz, R.E., Gray, D.J.: Organogenesis and somatic embryogenesis.-In: Hammerschlag, F.A., Litz, R.E. (ed.): Biotechnology of Perennial Fruit Crops. Pp. 3–34. University Press, Cambridge 1992.

    Google Scholar 

  • Lloyd, G., McCown, B.: Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture.-Proc. Int. Plant Propag. Soc. 30: 421–427, 1980.

    Google Scholar 

  • Lu, S.G., Bao, W.L., Meng, X.T.: Exploitation and ultilization of seabuckthorn in China.-In: Worldwide Research and Development of Seabuckthorn: Proceeding of International Workshop on Seabuckthorn, 1995. Pp. 1–8. China Sci-Tech Press, Beijing 1997.

    Google Scholar 

  • Merkie, S.A., Parrott, W.A., Flinn, B.S.: Morphogenic aspects of somatic embryogenesis.-In: Thorpe, T.A. (ed.): In Vitro Embryogenesis in Plant. Vol. 1. Pp. 3–8. Kluwer Academic Publishers, Dordrecht 1995.

    Google Scholar 

  • Montpetit, D., Lalonde, M.: In vitro propagation and subsequent nodulation of the actinorhizal Hippophae rhamnoides L.-Plant Cell Tissue Organ Cult. 15: 189–199, 1988.

    Article  CAS  Google Scholar 

  • Mou, Y.Y.: Micropropagation of seabuckthorn (Hippophae rhamnoides L.).-Agr. Sci. Finland 4: 503–512, 1995.

    Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bio-assays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Nitsch, J.P., Nitsch, C.: Haploid plants from pollen grains.-Science 163: 85–87, 1969.

    Article  PubMed  Google Scholar 

  • Palacios, A.M., Larrocea, M.P.O., Chávez, V.M., Bye, R.: Somatic embryogenesis of Agave victoriaereginae: considerations for its conservation.-Plant Cell Tissue Organ Cult. 74: 135–142, 2003.

    Article  Google Scholar 

  • Parrot, W.A., Bailey, M.A.: Characterization of recurrent somatic embryogenesis of alfalfa on auxin-free medium.-Plant Cell Tissue Organ Cult. 32: 69–76, 1993.

    Article  Google Scholar 

  • Raj, B.R.: Micropragation of temperate and tropical fruit trees through repetitive somatic embryogenesis.-In: XII Plant Tissue Culture Conference. Pp. 16. North-Eastern Hill University Press, Shillong 1989.

    Google Scholar 

  • Schenk, R.U., Hildebrandt, A.C.: Medium and techniques for induction and growth of monocotyledons and dicotyledonous plant cell cultures.-Can. J. Bot. 50: 199–204, 1972.

    Article  CAS  Google Scholar 

  • Soh, W.Y.: Germinability and cotyledon structure of somatic embryos in some dicotyledonous plants.-In: Xu, Z.H., Chen, Z.H. (ed.): Plant Biotechnology for Sustainable Development of Agriculture. Pp. 51–59. China Forestry Publishing House, Beijing 1996.

    Google Scholar 

  • Strickland, S.G., Nichol, J.W., McCall, C.M., Stuart, A.D.: Effect of carbohydrate source on alfalfa somatic embryogenesis.-Plant Sci. 48: 113–121, 1987.

    Article  CAS  Google Scholar 

  • Sun, Y.Q., Zhang, X.L., Jin, S.X., Liang, S.G., Nie, Y.C.: Somatic embryogenesis and plant regeneration in wild cotton (Gossypium klotzschianum).-Plant Cell Tissue Organ Cult. 75: 247–253, 2003.

    Article  CAS  Google Scholar 

  • Vieitez, E., Ballester, A., Vieitez, A.M.: Somatic embryogenesis and plant regeneration from cell suspension cultures of Fagus sylvatica L..-Hort. Rev. 14: 265–332, 1992.

    Google Scholar 

  • Vooková, B., Kormut’ák, A.: Effect of sucrose concentration, charcoal, and indole-3-butyric acid on germination of Abies numidica somatic embryos.-Biol. Plant. 44: 181–184, 2001.

    Article  Google Scholar 

  • Yang, H.P., Chen, J.C., Kamada, H.: Multiple pathways of somatic embryogenesis at a higher frequency in Asparagus officinalis L..-Plant Biotechnol. 17: 111–118, 2000.

    CAS  Google Scholar 

  • Yang, L.P., Zhang, F.L., Zhao, X.M.: [Rapid propagation of seabuckthorn (Hippophae rhamnoides L.).]-Global Seabuckthorn Res. Dev. 2: 12–16, 2004. [In Chin.]

    Google Scholar 

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Authors and Affiliations

  1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, P.R. China

    C. Q. Liu, X. L. Xia & W. L. Yin

  2. Graduate School, Beijing Forestry University, Beijing, 100083, P.R. China

    C. Q. Liu

  3. Phytopathology Key Laboratory, Yunnan Agricultural University, Kunming, 650201, Yunnan, P.R. China

    J. H. Zhou

  4. College of Forest and Horticulture, Sichuan Agricultural University, Yaan, 625014, Sichuan, P.R. China

    H. R. Tang

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  1. C. Q. Liu
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  2. X. L. Xia
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  3. W. L. Yin
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  4. J. H. Zhou
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Correspondence to W. L. Yin.

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Liu, C.Q., Xia, X.L., Yin, W.L. et al. Direct somatic embryogenesis from leaves, cotyledons and hypocotyls of Hippophae rhamnoides . Biol Plant 51, 635–640 (2007). https://doi.org/10.1007/s10535-007-0135-4

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  • DOI: https://doi.org/10.1007/s10535-007-0135-4

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