Abstract
A protocol for induction of direct somatic embryogenesis, secondary embryogenesis and plant regeneration of Dendrobium cv. Chiengmai Pink was developed. Thidiazuron (TDZ) at 0.3, 1 and 3 mg dm−3 induced 5–25 % of leaf tip segments of in vitro grown plants to directly form embryos after 60 d of culture, and 1 mg dm−3 TDZ was the best treatment. Somatic embryos mostly formed from leaf surfaces near cut ends, and occasionally found on leaf tips. Higher frequency of embryogenesis was obtained in light than in darkness. During subculture, secondary embryos developed from outer cell layers of primary embryos. All combinations of NAA (0, 0.1, 1 mg dm−3) and TDZ (0, 0.3, 1, 3 mg dm−3) increased the multiplication rate of embryos. It takes about 8 months from embryo induction, plantlet formation to eventually acclimatization in greenhouse.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- MS:
-
Murashige and Skoog (1962) medium
- NAA:
-
naphthaleneacetic acid
- TDZ:
-
thidiazuron, 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea
References
Arditti, J., Ernst, R. (ed.): Micropropagation of Orchids. Pp. 467–520. John Wiley & Sons, New York 1993.
Chen, J.T., Chang, W.C.: Effects of auxins and cytokinins on direct somatic embryogenesis from leaf explants of Oncidium ‘Gower Ramsey’.-Plant Growth Regul. 34: 229–232, 2001.
Chen, J.T., Chang, W.C.: Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis.-Biol. Plant. 50: 169–173, 2006.
Chen, J.T., Chang, C., Chang, W.C.: Direct somatic embryogenesis from leaf explants of Oncidium ‘Gower Ramsey’ and subsequent plant regeneration.-Plant Cell Rep. 19: 143–149, 1999.
Dawns, C.J.: Biological Techniques in Electron Microscopy.-Barnes and Noble, New York 1971.
Duncan, D.B.: Multiple range and multiple F test.-Biometrics 11: 1–42, 1955.
Jensen, W.A.: Botanical Histochemistry.-Freeman, San Francisco 1962.
Manorama, P., Rao, A.N., Goh, C.J., Loh, C.S.: Leaf callus development in Aranda and Dendrobium.-In: Fifth ASEAN Orchid Congress. Pp. 102–109. National University of Singapore, Singapore 1984.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant 15: 495–497, 1962.
Nayak, N.R., Rath, S.P., Patnaik, S.: In vitro propagation of three epiphytic orchids, Cymbidium aloifolium (L.) Sw., Dendrobium aphyllum (Roxb.) Fisch and Dendrobium moschatum (Buch-Ham) Sw. through thidiazuron-induced high frequency shoot proliferation.-Sci. Hort. 71: 243–250, 1997.
Nayak, N.R., Sahoo, S., Patnaik, S., Rath, S.P.: Establishment of thin cross section (TCS) culture method for rapid micropropagation of Cymbidium aloifolium (L.) Sw. and Dendrobium nobile Lindl. var. oculatum Hk. f.-Sci. Hort. 94: 107–116, 2002.
Teob, E.S. (ed.): Orchids of Asia.-Times Books International, Singapore 1989.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chung, H.H., Chen, J.T. & Chang, W.C. Plant regeneration through direct somatic embryogenesis from leaf explants of Dendrobium . Biol Plant 51, 346–350 (2007). https://doi.org/10.1007/s10535-007-0069-x
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-007-0069-x