Abstract
The effect of excessive Cd on the growth and metal uptake by leafy vegetables Brassica chinensis L. (cv. Wuyueman) and Brassica pekinensis (Lour.) Rupr. (cv. Qingyan 87-114) were studied in hydroponic solution culture. The Cd concentration higher than 10 µM significantly decreased the root elongation and leaf chlorophyll contents of both plant species. The shoots of B. pekinensis had significantly higher concentrations of total and water-soluble Cd than B. chinensis. The roots of both species accumulated more Cd than the shoots in all the Cd treatments. Most of the Cd in the roots was found in the cell walls. The shoot/root ratio of Cd concentrations in B. pekinensis was always greater than that in B. chinensis. But, the concentration and proportion of Cd in the cell walls in B. chinensis were higher than that in B. pekinensis. Cadmium treatments also increased the concentrations of total non-protein thiols (NPT) in the shoots of the both species. A significant correlation was found between the concentrations of soluble Cd and NPT in plant shoots.
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Abbreviations
- GSH:
-
glutathion
- NPT:
-
non-protein thiols
- PC:
-
phytochelatins
References
Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1–15, 1949.
Baker, A.J.M.: Metal tolerance.-New Phytol. 106: 93–111, 1987.
Baker, A.J.M., Brooks, R.R.: Terrestrial higher plants which hyperaccumulate metallic elements — a review of their distribution, ecology and phytochemistry.-Biorecovery 1: 81–126, 1989.
Cakmak, I., Marschner, H.: Enhanced superoxide radical production in roots of zinc deficient plants.-J. exp. Bot. 39: 1449–1460, 1988.
Carrier, P., Baryla, A., Havaux, M.: Cadmium distribution and microlocalization in oilseed rape (Brassica napus) after long-term growth on cadmium-contaminated soil.-Planta 216: 939–950, 2003.
Ellman, G.L.: Tissue sulfhydryl groups.-Arch. Biochem. Biophys. 82: 70–77, 1959.
Hall, J.L.: Cellular mechanisms for heavy metal detoxification and tolerance.-J. exp. Bot. 53: 1–11, 2002.
Hart, J.J., DiTomaso, J.M., Linscott, D.L., Kochian, L.V.: Characterization of the transport and cellular compartmentation of paraquat in roots of intact maize seedlings.-Pestic. Biochem. Physiol. 43: 212–222, 1992.
Howden, R., Anderson, C.R., Goldsbrough, P.B., Cobbett, C.S.: A cadmium-sensitive, glutathione-deficient mutant of Arabidopsis thaliana.-Plant Physiol. 107: 1067–1073, 1995.
Kneer, R., Zenk, M.H.: Phytochelatins protect plant enzymes from heavy metal poisoning.-Phytochemistry 31: 2663–2667, 1992.
Kumar, P.B.A.N., Dushenkov, V., Motto, H., Raskin, I.: Phytoextraction: the use of plants to remove heavy metals from soils.-Environ. Sci. Technol. 29: 1232–1238, 1995.
Lou, L.Q., Shen, Z.G., Li, X.D.: The copper tolerance mechanisms of Elsholtzia haichowensis, a plant from copper-enriched soils.-Environ. exp. Bot. 51: 111–120, 2004.
Lucarini, M., Canali, R., Cappelloni, M., Di Lullo, G., Lombardi-Boccia, G..: In vitro calcium availability from Brassica vegetables (Brassica oleracea L.) and as consumed in composite dishes.-Food Chem. 64: 519–523, 1999.
Macfie, S.M., Welbourn, P.M.: The cell wall as a barrier to uptake of metal ions in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae).-Arch. Environ. Contamin. Toxicol. 39: 413–419, 2000.
Pandey, N., Sharma, C.P.: Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth and metabolism of cabbage.-Plant Sci. 163: 753–758, 2002.
Ramos, I., Esteban, E., Lucena, J.J., Gárate, A.: Cadmium uptake and subcellular distribution in plants of Lactuca sp. Cd-Mn interaction.-Plant Sci. 162: 761–767, 2002.
Rauser, W.E.: Phytochelatins and related peptides — structure, biosynthesis, and function.-Plant Physiol. 109: 1141–1149, 1995.
Reeves, R.D., Baker, A.J.M.: Metal-accumulating plants.-In: Raskin, I., Ensley, B.D. (ed.): Phytoremediation of Toxic Metals: Using Plants to Clean up the Environment. Pp. 193–229. John Wiley & Sons, New York 2000.
Ru, S.H., Wang, J.Q., Su, D.C.: Characteristics of Cd uptake and accumulation in two Cd accumulator oilseed rape species.-J. environ Sci. China 16: 594–598, 2004.
Salt, D.E., Prince, R.C., Pickering, I.J., Raskin, I.: Mechanisms of cadmium mobility and accumulation in Indian mustard.-Plant Physiol. 109: 1427–1433, 1995.
Sanità di Toppi, L., Gabbrielli, R.: Response to cadmium in higher plants.-Environ. exp. Bot. 41: 105–130, 1999.
Scandalios J.G.: Oxygen stress and superoxide dismutase.-Plant Physiol. 101: 7–12, 1993.
Skórzyńska-Polit, E., Drążkiewicz, M., Krupa, Z.: The activity of the antioxidative system in cadmium-treated Arabidopsis thaliana.-Biol. Plant. 47: 71–78, 2003/4.
Stolt, J.P., Sneller, F.E.C., Bryngelsson, T., Lundborg, T., Schat, H.: Phytochelatin and cadmium accumulation in wheat.-Environ. exp. Bot. 49: 21–28, 2003.
Van Assche, F., Clijsters, H.: Effects of metals on enzyme activity in plants.-Plant Cell Environ. 13: 195–206, 1990.
Vassilev, A., Lidon, F., Scotti, P., Da Graca, M., Yordanov, I.: Cadmium-induced changes in chloroplast lipids and photosystem activities in barley plants.-Biol. Plant. 48: 153–156, 2004.
Wagner, G.J.: Accumulation of cadmium in crop plants and its consequences to human health.-Adv. Agron. 51: 173–212, 1993.
Wójcik, M., Vangronsveld, J., D’Haen, J., Tukiendorf, A.: Cadmium tolerance in Thlaspi caerulescens. II. Localization of cadmium in Thlaspi caerulescens.-Environ. exp. Bot. 53: 163–171, 2005.
Zhang, F.Q., Li, X.D., Wang, C.C., Shen, Z.G.: Effect of cadmium on autoxidation rate of tissue and inducing accumulation of free proline in seedlings mung bean.-J. Plant Nutr. 23: 357–368, 2000.
Zhang, F.Q., Shi, W.Y., Jin, Z.X., Shen, Z.G.: Response of antioxidative enzymes in cucumber chloroplasts to cadmium toxicity.-J. Plant Nutr. 26: 1779–1788, 2003.
Zornoza, P., Vázquez, S., Esteban, E., Fernández-Pascual, M., Carpena, R.: Cadmium-stress in nodulated white lupin: strategies to avoid toxicity.-Plant Physiol Biochem. 40: 1003–1009, 2002.
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Liu, C.P., Shen, Z.G. & Li, X.D. Accumulation and detoxification of cadmium in Brassica pekinensis and B. chinensis . Biol Plant 51, 116–120 (2007). https://doi.org/10.1007/s10535-007-0023-y
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DOI: https://doi.org/10.1007/s10535-007-0023-y