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Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton

  • Original Paper
  • Published:
Biologia Plantarum

Abstract

Hypocotyls of cotton (Gossypium hirsutum L.) cultivars cv. YZ-1, Coker 312 and Coker 201 were inoculated on Murashige and Skoog callus induction medium. YZ-1 exhibited a very high regeneration potential, with 81.9 % of the explants inoculated differentiated into embryogenic callus within 8–10 weeks. During the process of callus maintenance (subculture for 1 to 3 years), the total embryos number in Coker 312 and Coker 201 calli dropped sharply, and the percentage of embryo germination decreased. On the contrary, the callus of YZ-1 consistently maintains a high frequency of plant regeneration after long-time subculture. Transgenic kanamycin-resistant calli of Coker 201 partially lost the ability of somatic embryogenesis and plant regeneration. The stress produced by the transformation procedure slightly affected somatic embryogenesis and plant regeneration of YZ-1, which showed minimum loss of plant regeneration ability.

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Abbreviations

EC:

embryogenic callus

MSB:

MS medium (Murashige and Skoog 1962) and B5 (Gamborg et al. 1968) vitamins

IBA:

indole-3-butyric acid

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

α-naphthalene acetic acid

npt II:

neomycin phosphotransferase

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, P.R. China

    S. Jin, X. Zhang, Y. Nie, X. Guo, S. Liang & H. Zhu

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  1. S. Jin
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  2. X. Zhang
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  3. Y. Nie
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  4. X. Guo
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  5. S. Liang
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  6. H. Zhu
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Correspondence to X. Zhang.

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Jin, S., Zhang, X., Nie, Y. et al. Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton. Biol Plant 50, 519–524 (2006). https://doi.org/10.1007/s10535-006-0082-5

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  • DOI: https://doi.org/10.1007/s10535-006-0082-5

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