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Agrobacterium tumefaciens-mediated transformation of safflower (Carthamus tinctorius L.) cv. ‘Centennial’

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Abstract

Efficient callus formation was achieved from cotyledon, stem, and leaf expiants of the domestic safflower cultivar ‘Centennial’ on MS salts medium containing 1 mg/L BAP and 1 mg/L NAA. Shoot buds were regenerated from 26% of leaf-derived calli on callus induction medium, although attempts to root regenerated shoots were not successful. ‘Centennial’ expiants inoculated with Agrobacterium tumefaciens containing NPT II and GUS genes produced kanamycin-resistant calli from which buds were regenerated. Transformation and stable integration of transgenes was confirmed by GUS assay and DNA hybridization in kanamycin-resistant calli, and GUS assay in regenerated shoots.

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Abbreviations

BAP:

6-benzylaminopurine

NAA:

1-naphthaleneacetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

MS:

Murashige and Skoog (1962)

GUS:

β-glucuronidase

IAA:

indole-3-acetic acid

NPT II:

neomycin phosphotransferase II

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Author notes

  1. Miaocheng Ying

    Present address: Aberdeen Research and Extension Center, University of Idaho, 83210, Aberdeen, Idaho, USA

  2. Jerald W. Bergman

    Present address: Eastern Agricultural Research Center, Montana State University, P.O. Box 1350, 59270, Sidney, Montana, USA

Authors and Affiliations

  1. Plant and Soil Science Department, Montana State University, 59717, Bozeman, Montana, USA

    Miaocheng Ying, William E. Dyer & Jerald W. Bergman

Authors
  1. Miaocheng Ying
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  2. William E. Dyer
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  3. Jerald W. Bergman
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Communicated by G. C. Phillips

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Ying, M., Dyer, W.E. & Bergman, J.W. Agrobacterium tumefaciens-mediated transformation of safflower (Carthamus tinctorius L.) cv. ‘Centennial’. Plant Cell Reports 11, 581–585 (1992). https://doi.org/10.1007/BF00233097

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  • DOI: https://doi.org/10.1007/BF00233097

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