Abstract
A modified plant 7SL RNA gene from Arabidopsis thaliana designated AHIIA63M was introduced into potato plants via Agrobacterium-mediated transformation. No transgenic plants could be obtained using pGPTV-based binary vectors where AHIIA63M gene driven by polIII promoter was located close to the polII promoter of the selection gene. Special binary vectors with matrix attachment region (MAR) elements had to be used for transformation to insulate polII and polIII promoters within T-DNA. The level of AHIIA63M RNA in transgenic plants was lower than the levels of transcripts of transgenes driven by RNA polymerase II. The level of AHIIA63M transcript in transgenic potato plants was tissue specific. The highest expression was detected in roots and gynoecium and the lowest in tubers. Moreover, non-specific promoter activity within the MAR element was revealed. This activity contributed to AHIIA63M transcription. This is the first report of expression of a modified 7SL RNA gene in transgenic plants and promoter activity within the MAR element.
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Abbreviations
- A element:
-
chicken lysozyme A element
- GUS:
-
β-glucuronidase
- MAR:
-
matrix attachment region
- NPTII:
-
neomycin phosphotransferase II
- P-35S:
-
cauliflower mosaic virus 35S promoter
- P-nos:
-
nopaline synthase promoter
- polII:
-
RNA polymerase II
- polIII:
-
RNA polymerase III
- SRP:
-
signal recognition particle
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This work was supported by grants MSMT WTZ CZE 00/003 and 02/032. The stay of L. Vrba at Plant Research International in Wageningen, Netherlands, was supported by the EMBO Short term fellowship ASTF 9513.
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Vrba, L., Matousek, J. Expression of modified 7SL RNA gene in transgenic Solanum tuberosum plants. Biol Plant 49, 371–380 (2005). https://doi.org/10.1007/s10535-005-0010-0
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DOI: https://doi.org/10.1007/s10535-005-0010-0