Abstract
In order to systematically perform an experimental and theoretical study on DNA binding and photocleavage properties of transition metal complexes of the type [M(L)2(L1)](PF6)n · xH2O (where M = Co(III) or Ni(II), L = 1,10-phenanthroline or 2.2′ bipryidine, L1 = Thiophene [2,3-b] quinoline (qt), n = 3 or 2 and x = 5 or 2) have been synthesized and characterized by elemental analysis, IR, 1H NMR, UV and magnetic susceptibility data. The DNA-binding properties of these complexes have been investigated with UV-Vis, viscosity measurements, thermal denaturation and cyclic voltametric studies. It is experimentally found that all the complexes are bound to DNA via intercalation in the order [Co(bpy)2(qt)](PF6)3 > [Co(phen)2(qt)](PF6)3 > [Ni(phen)2(qt)](PF6)2 > [Ni(bpy)2(qt)](PF6)2. The photocleavage studies with pUC19 DNA shows that all these complexes promoted the conversion of SC form to NC form in absence of ‘inhibitors’.
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Abbreviations
- SC:
-
Super coiled
- NC:
-
Nicked circular
- DNA:
-
Deoxyribose nucleic acid
- pUC:
-
plasmid University of California
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One of the author Prabhakara M.C. wish to thank Kuvempu University for providing Senior Research Fellowship and the Director, SIFC, IISc. Bangalore for Spectral data.
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Prabahkara, M.C., Bhojya Naik, H.S. Binding and photocleavage of DNA by mixed ligand Co(III) and Ni(II) complexes of thiophene[2, 3-b] quinoline and phenanthrolie/bipyridine. Biometals 21, 675–684 (2008). https://doi.org/10.1007/s10534-008-9152-9
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DOI: https://doi.org/10.1007/s10534-008-9152-9