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Characterization of a Mn-dependent Fructose-1,6-bisphosphate Aldolase in Deinococcus radiodurans

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Abstract

The key enzyme of the glycolytic pathway of Deinococcus radiodurans, fructose-1,6-bisphosphate aldolase, could be induced independently by glucose and Mn. The enzyme exhibited the characteristics of the metal-dependent Class II aldolases. Unlike most Class II aldolases, the deinococcal aldolase preferred Mn, not Zn, as a cofactor. The fbaA gene encoding the deinococcal aldolase was cloned and the protein overproduced in various Escherichia coli expression hosts. However, the overexpressed deinococcal enzyme aggregated and formed inclusion bodies. Dissolving these inclusion bodies by urea and subsequent purification by nickel affinity chromatography, resulted in a protein fraction that exhibited aldolase activity only in the presence of Mn. This active aldolase fraction exhibited masses of about 70 kDa and 35 kDa by gel filtration and by SDS gel electrophoresis, respectively, suggesting that the active aldolase was a dimer.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Cell Sciences, University of Memphis, Memphis, TN, 38152, USA

    Yong-Mei Zhang & Tit-Yee Wong

  2. Department of Biological Sciences, National Sun Yat-sen University, Taiwan, Republic of China

    Jong-Kang Liu

  3. Department of Anatomy & Neurobiology, University of Tennessee Health Science Center, Memphis, TN, 38163, USA

    Mohammad Reza Shouri

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  1. Yong-Mei Zhang
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  2. Jong-Kang Liu
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  3. Mohammad Reza Shouri
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  4. Tit-Yee Wong
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Correspondence to Tit-Yee Wong.

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Zhang, YM., Liu, JK., Shouri, M.R. et al. Characterization of a Mn-dependent Fructose-1,6-bisphosphate Aldolase in Deinococcus radiodurans. Biometals 19, 31–37 (2006). https://doi.org/10.1007/s10534-005-4320-7

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  • DOI: https://doi.org/10.1007/s10534-005-4320-7

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