Abstract
Accumulation of plastic waste has become an environmental threat and a global problem. In this research, polyethylene degrading ligninolytic bacteria were isolated from plastic waste polluted soil. Two bacterial isolates, namely PE2 and PE3 have been obtained from the soil samples. Polyethylene degrading ability of the isolates has been assessed individually in a synthetic media containing polyethylene as a carbon source. The results indicated that maximum weight reduction of polyethylene (6.68%) was found in PE3 inoculated media after thirty days of incubation. Fourier Transform Infrared Spectroscopic results showed the appearance of carbonyl peaks. 16S rRNA gene sequencing studies revealed that the potential isolate PE3 belongs to the genus Bacillus and it was named Bacillus sp. strain PE3. From the scanning electron microscopic results, it is inferred that Bacillus sp. strain PE3 could colonize on the polyethylene surface and form a biofilm. Besides, the viable Bacillus sp. strain PE3 on polyethylene surface was confirmed by fluorescence microscopic analysis. Alkanes and fatty acids were identified in the degraded products by gas chromatography-mass spectrometer analysis. From the results of native polyacrylamide gel electrophoresis, the activities of laccase and lignin peroxidase were noticed. Furthermore, extracellular production of biosurfactant has been observed in the Bacillus sp. strain PE3 inoculated mineral salt media and synthetic media with glucose and polyethylene as the carbon source respectively. The characterization studies of crude biosurfactant have confirmed that lipopeptide nature biosurfactant. The present study demonstrates that the ligninolytic enzymes laccase, lignin peroxidase, and lipopeptide type biosurfactant are produced by Bacillus sp. strain PE3 in the media with polyethylene as a carbon source.
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Acknowledgements
This work was partially supported by the Rastriya Uchchatar Shiksha Abhiyan (RUSA) 2.0 Bharathiar University Entrepreneur, Innovation and Career Hub (BEICH), Ministry of Human Resource Development (MHRD), Department of Higher Education, Government of India, India, under Grant BU/ RUSA/ BEICH/2019/299-41. The authors express their gratitude for the support extended by the authorities of the Department of Biochemistry, Biotechnology, and Bioinformatics, Avinashilingam Institute for Home Science and Higher education for women, Coimbatore. Authors would like to thank Dr. K. Suresh, Assistant Professor, Department of Physics, Bharathiar University for the help received to improve FTIR spectral studies.
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Kavitha, R., Bhuvaneswari, V. Assessment of polyethylene degradation by biosurfactant producing ligninolytic bacterium. Biodegradation 32, 531–549 (2021). https://doi.org/10.1007/s10532-021-09949-8
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DOI: https://doi.org/10.1007/s10532-021-09949-8