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Diesel oil removal by immobilized Pseudoxanthomonas sp. RN402

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Abstract

Pseudoxanthomonas sp. RN402 was capable of degrading diesel, crude oil, n-tetradecane and n-hexadecane. The RN402 cells were immobilized on the surface of high-density polyethylene plastic pellets at a maximum cell density of 108 most probable number (MPN) g−1 of plastic pellets. The immobilized cells not only showed a higher efficacy of diesel oil removal than free cells but could also degrade higher concentrations of diesel oil. The rate of diesel oil removal by immobilized RN402 cells in liquid culture was 1,050 mg l−1 day−1. Moreover, the immobilized cells could maintain high efficacy and viability throughout 70 cycles of bioremedial treatment of diesel-contaminated water. The stability of diesel oil degradation in the immobilized cells resulted from the ability of living RN402 cells to attach to material surfaces by biofilm formation, as was shown by CLSM imaging. These characteristics of the immobilized RN402 cells, including high degradative efficacy, stability and flotation, make them suitable for the purpose of continuous wastewater bioremediation.

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Acknowledgments

This work was supported by grants from the Office of the National Research Council of Thailand, the Office of the Higher Education Commission, Thailand, the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund), Chulalongkorn University, and the Thai Government Stimulus Package 2 (TKK2555) under the Project for the Establishment of the Comprehensive Center for Innovative Food, Health Products and Agriculture.

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Authors and Affiliations

  1. Inter-Department of Environmental Science, Graduate School, Chulalongkorn University, Bangkok, 10330, Thailand

    Wannarak Nopcharoenkul

  2. Bioremediation Research Unit, Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Parichat Netsakulnee & Onruthai Pinyakong

  3. Center of Excellence for Environmental and Hazardous Waste Management (EHWM), Bangkok, 10330, Thailand

    Onruthai Pinyakong

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  1. Wannarak Nopcharoenkul
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  2. Parichat Netsakulnee
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  3. Onruthai Pinyakong
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Correspondence to Onruthai Pinyakong.

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Nopcharoenkul, W., Netsakulnee, P. & Pinyakong, O. Diesel oil removal by immobilized Pseudoxanthomonas sp. RN402. Biodegradation 24, 387–397 (2013). https://doi.org/10.1007/s10532-012-9596-z

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