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Production of thermotolerant and alkalotolerant cellulolytic enzymes by isolated Nocardiopsis sp. KNU

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Abstract

A novel cellulolytic bacterium was isolated from the forest soil of KNU University campus. Through 16S rRNA sequence matching and morphological observation it was identified as Nocardiopsis sp. KNU. This strain can utilize a broad range of cellulosic substrates including: carboxymethyl cellulose (CMC), avicel, xylan, cellobiose, filter paper and rice straw by producing a large amount of thermoalkalotolerant endoglucanase, exoglucanase, xylanase and glucoamylase. Optimal culture conditions (Dubos medium, 37°C, pH 6.5 and static condition) for the maximal production of the cellulolytic enzymes were determined. The activity of cellulolytic and hemicelluloytic enzymes produced by this strain was mainly present extracellularly and the enzyme production was dependent on the cellulosic substrates used for the growth. Effect of physicochemical conditions and metal additives on the cellulolytic enzymes production were systematically investigated. The cellulases produced by Nocardiopsis sp. KNU have an optimal temperature of 40°C and pH of 5.0. These cellulases also have high thermotolerance as evidenced by retaining 55–70% activity at 80°C and pH of 5.0 and alkalotolerance by retaining >55% of the activity at pH 10 and 40°C after 1 h. The efficiency of fermentative conversion of the hydrolyzed rice straw by Saccharomyces cerevisiae (KCTC-7296) resulted in 64% of theoretical ethanol yield.

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Acknowledgments

This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2008-314-F00014). The authors would like to acknowledge the technical help from Prof. Kim M. D., Mr. Yoo J. G. and Mr. Woo Chang, Kangwon National University, South Korea. One of the author (GDS) would like to thank to Prof. S. P. Govindwar, Head, Department of Biochemistry, Shivaji University, India for their support and encouragement.

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Authors and Affiliations

  1. Department of Biochemistry, Shivaji University, Kolhapur, Maharashtra, 416004, India

    Ganesh D. Saratale

  2. Bioenergy Laboratory, Department of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwondo, 200-701, South Korea

    Ganesh D. Saratale & Sang Eun Oh

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  1. Ganesh D. Saratale
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  2. Sang Eun Oh
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Correspondence to Sang Eun Oh.

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Saratale, G.D., Oh, S.E. Production of thermotolerant and alkalotolerant cellulolytic enzymes by isolated Nocardiopsis sp. KNU. Biodegradation 22, 905–919 (2011). https://doi.org/10.1007/s10532-010-9450-0

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  • DOI: https://doi.org/10.1007/s10532-010-9450-0

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