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Effects of enrichment with phthalate on polycyclic aromatic hydrocarbon biodegradation in contaminated soil

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Abstract

The effect of enrichment with phthalate on the biodegradation of polycyclic aromatic hydrocarbons (PAH) was tested with bioreactor-treated and untreated contaminated soil from a former manufactured gas plant (MGP) site. Soil samples that had been treated in a bioreactor and enriched with phthalate mineralized 14C-labeled phenanthrene and pyrene to a greater extent than unenriched samples over a 22.5-h incubation, but did not stimulate benzo[a]pyrene mineralization. In contrast to the positive effects on 14C-labeled phenanthrene and pyrene, no significant differences were found in the extent of biodegradation of native PAH when untreated contaminated soil was incubated with and without phthalate amendment. Denaturing-gradient gel electrophoresis (DGGE) profiles of bacterial 16S rRNA genes from unenriched and phthalate-enriched soil samples were substantially different, and clonal sequences matched to prominent DGGE bands revealed that β-Proteobacteria related to Ralstonia were most highly enriched by phthalate addition. Quantitative real-time PCR analyses confirmed that, of previously determined PAH-degraders in the bioreactor, only Ralstonia-type organisms increased in response to enrichment, accounting for 89% of the additional bacterial 16S rRNA genes resulting from phthalate enrichment. These findings indicate that phthalate amendment of this particular PAH-contaminated soil did not significantly enrich for organisms associated with high molecular weight PAH degradation or have any significant effect on overall degradation of native PAH in the soil.

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Acknowledgments

We thank Chad Roper for assistance in the quantification of PAH by HPLC. We also thank Wei Sun of the UNC Department of Biostatistics for helpful discussions concerning data analyses. This work was supported by the National Institute of Environmental Health Sciences (grant number 5 P42 ES05948).

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  1. Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, NC, 27599, USA

    David R. Singleton, Stephen D. Richardson & Michael D. Aitken

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  1. David R. Singleton
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  2. Stephen D. Richardson
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  3. Michael D. Aitken
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Correspondence to David R. Singleton.

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Singleton, D.R., Richardson, S.D. & Aitken, M.D. Effects of enrichment with phthalate on polycyclic aromatic hydrocarbon biodegradation in contaminated soil. Biodegradation 19, 577–587 (2008). https://doi.org/10.1007/s10532-007-9163-1

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  • DOI: https://doi.org/10.1007/s10532-007-9163-1

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