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Obtaining hemicellulosic hydrolysate from sugarcane bagasse for microbial oil production by Lipomyces starkeyi

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Abstract

Objective

The extraction of the hemicellulose fraction of sugarcane bagasse (SCB) by acid hydrolysis was evaluated in an autoclave and a Parr reactor aiming the application of the hydrolysate as a carbon source for lipid production by Lipomyces starkeyi.

Results

The hydrolysis that resulted in the highest sugar concentration was obtained by treatment in the Parr reactor (HHR) at 1.5% (m/v) H2SO4 and 120 °C for 20 min, reaching a hemicellulose conversion of approximately 82%. The adaptation of the yeast to the hydrolysate provided good fermentability and no lag phase. The fermentation of hemicellulose-derived sugars (HHR) by L. starkeyi resulted in a 27.8% (w/w) lipid content and YP/S of 0.16 g/l.h. Increasing the inoculum size increased the lipid content by approximately 61%, reaching 44.8% (w/w).

Conclusion

The hemicellulose hydrolysate from SCB is a potential substrate for L. starkeyi to produce lipids for biodiesel synthesis based on the biorefinery concept.

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Acknowledgments

We would like to thank FAPESP (Foundation for Research Support of the State of São Paulo), CAPES (Coordination for the Improvement of Higher Education Personnel) and CNPq (National Council for Scientific and Technological Development) for their support of this research. We would like to thank LNBR (Brazilian Biorenewables National Laboratory) and Dr. Carlos Vaz Rossel for providing the sugarcane bagasse and the Parr reactor.

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  1. Department of Bioprocess Engineering and Biotechnology, Federal University of Tocantins (UFT), Badejos Street 69-72, Jardim Cervilha, Gurupi, TO, 77404-970, Brazil

    Michelle da Cunha Abreu Xavier

  2. Department of Process Engineering (DEPro), School of Chemical Engineering, State University of Campinas (UNICAMP), Albert Einstein Avenue, 500, Zeferino Vaz University City, Campinas, SP, 13083-852, Brazil

    Telma Teixeira Franco

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Correspondence to Michelle da Cunha Abreu Xavier.

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da Cunha Abreu Xavier, M., Teixeira Franco, T. Obtaining hemicellulosic hydrolysate from sugarcane bagasse for microbial oil production by Lipomyces starkeyi. Biotechnol Lett 43, 967–979 (2021). https://doi.org/10.1007/s10529-021-03080-7

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