Skip to main content
SpringerLink
Log in

Enhanced pyruvate production in Candida glabrata by overexpressing the CgAMD1 gene to improve acid tolerance

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Objectives

To enhance acid tolerance of Candida glabrata for pyruvate production by engineering AMP metabolism.

Results

The physiological function of AMP deaminase in AMP metabolism from C. glabrata was investigated by deleting or overexpresseing the corresponding gene, CgAMD1. At pH 4, CgAMD1 overexpression resulted in 59 and 51% increases in biomass and cell viability compared to those of wild type strain, respectively. In addition, the intracellular ATP level of strain Cgamd1Δ/CgAMD1 was down-regulated by 22%, which led to a 94% increase in pyruvate production. Further, various strengths of CgAMD1 expression cassettes were designed, thus resulting in a 59% increase in pyruvate production at pH 4. Strain Cgamd1Δ/CgAMD1 (H) was grown in a 30 l batch bioreactor at pH 4, and pyruvate reached 46.1 g/l.

Conclusion

CgAMD1 overexpression plays an active role in improving acid tolerance and pyruvate fermentation performance of C. glabrata at pH 4.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Canelas AB, ten Pierick A, Ras C, Seifar RM, van Dam JC, van Gulik WM, Heijnen JJ (2009) Quantitative evaluation of intracellular metabolite extraction techniques for yeast metabolomics. Anal Chem 81:7379–7389

    Article  CAS  PubMed  Google Scholar 

  • Chen X, Song W, Gao C, Qin W, Luo Q, Liu J, Liu L (2016) Fumarate production by Torulopsis glabrata: engineering heterologous fumarase expression and improving acid tolerance. PLoS ONE 11:e0164141

    Article  PubMed  PubMed Central  Google Scholar 

  • Hida H, Yamada T, Yamada Y (2007) Genome shuffling of Streptomyces sp. U121 for improved production of hydroxycitric acid. Appl Microbiol Biotechnol 73:1387–1393

    Article  CAS  PubMed  Google Scholar 

  • Hua Q, Joyce AR, Fong SS, Palsson BO (2006) Metabolic analysis of adaptive evolution for in silico-designed lactate-producing strains. Biotechnol Bioeng 95:992–1002

    Article  CAS  PubMed  Google Scholar 

  • Itoh R, Saint-Marc C, Chaignepain S, Katahira R, Schmitter JM, Daignan-Fornier B (2003) The yeast ISN1 (YOR155c) gene encodes a new type of IMP-specific 5′-nucleotidase. BMC Biochem 4:4

    Article  PubMed  PubMed Central  Google Scholar 

  • Liu L, Li Y, Du G, Chen J (2006a) Redirection of the NADH oxidation pathway in Torulopsis glabrata leads to an enhanced pyruvate production. Appl Microbiol Biotechnol 72:377–385

    Article  CAS  PubMed  Google Scholar 

  • Liu L, Li Y, Li H, Chen J (2006b) Significant increase of glycolytic flux in Torulopsis glabrata by inhibition of oxidative phosphorylation. FEMS Yeast Res 6:1117–1129

    Article  CAS  PubMed  Google Scholar 

  • Loret MO, Pedersen L, Francois J (2007) Revised procedures for yeast metabolites extraction: application to a glucose pulse to carbon-limited yeast cultures, which reveals a transient activation of the purine salvage pathway. Yeast 24:47–60

    Article  CAS  PubMed  Google Scholar 

  • Morrison BA, Shain DH (2008) An AMP nucleosidase gene knockout in Escherichia coli elevates intracellular ATP levels and increases cold tolerance. Biol Lett 4:53–56

    Article  CAS  PubMed  Google Scholar 

  • Parry BR, Shain DH (2011) Manipulations of AMP metabolic genes increase growth rate and cold tolerance in Escherichia coli: implications for psychrophilic evolution. Mol Biol Evol 28:2139–2145

    Article  CAS  PubMed  Google Scholar 

  • Schmid A, Dordick JS, Hauer B, Kiener A, Wubbolts M, Witholt B (2001) Industrial biocatalysis today and tomorrow. Nature 409:258–268

    Article  CAS  PubMed  Google Scholar 

  • Xu G, Liu L, Chen J (2012) Reconstruction of cytosolic fumaric acid biosynthetic pathways in Saccharomyces cerevisiae. Microb Cell Fact 11:24

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhou J, Liu L, Shi Z, Du G, Chen J (2009) ATP in current biotechnology: regulation, applications and perspectives. Biotechnol Adv 27:94–101

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

This study was supported by the National Natural Science Foundation of China (21576117, 21706095), the Key Technologies R&D Program of Jiangsu Province (BE2015307, BE2017622), the Fundamental Research Funds for the Central Universities (JUSRP51611A) and the National Science Foundation for Post-doctoral Scientists of China (2016M600362).

Supporting information

Supplementary Table 1—Strains and plasmids used.

Author information

Authors and Affiliations

  1. School of Pharmaceutical Science, Jiangnan University, Wuxi, 214122, China

    Jing Wu & Qiuling Luo

  2. State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China

    Qiuling Luo, Jia Liu, Xiulai Chen & Liming Liu

  3. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Xiulai Chen & Liming Liu

Authors
  1. Jing Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qiuling Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jia Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiulai Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Liming Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiulai Chen.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 27 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wu, J., Luo, Q., Liu, J. et al. Enhanced pyruvate production in Candida glabrata by overexpressing the CgAMD1 gene to improve acid tolerance. Biotechnol Lett 40, 143–149 (2018). https://doi.org/10.1007/s10529-017-2452-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-017-2452-9

Keywords

Search

Navigation