Abstract
Objectives
To evaluate the crystallinity index of the cellulose produced by Escherichia coli Nissle 1917 after heterologous expression of the cellulose synthase subunit D (bcsD) gene of Gluconacetobacter xylinus BPR2001.
Results
The bcsD gene of G. xylinus BPR2001 was expressed in E. coli and its protein product was visualized using SDS-PAGE. FTIR analysis showed that the crystallinity index of the cellulose produced by the recombinants was 0.84, which is 17% more than that of the wild type strain. The increased crystallinity index was also confirmed by X-ray diffraction analysis. The cellulose content was not changed significantly after over-expressing the bcsD.
Conclusion
The bcsD gene can improve the crystalline structure of the bacterial cellulose but there is not any significant difference between the amounts of cellulose produced by the recombinant and wild type E. coli Nissle 1917.
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Acknowledgements
The National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran supported this work. The authors would like to thank Dr. Atefeh Sharifirad, Mr. Sina Mehrpooyan, Dr. Mehdi Mohammadi, and Ms. Faezeh Aliasgari for their valuable comments and supports.
Supporting information
Additional file 1—DNA manipulations.
Additional file 2—Expression of BcsD protein.
Additional file 3—Characterizing cellulose production by Congo Red.
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Sajadi, E., Babaipour, V., Deldar, A.A. et al. Enhancement of crystallinity of cellulose produced by Escherichia coli through heterologous expression of bcsD gene from Gluconacetobacter xylinus . Biotechnol Lett 39, 1395–1401 (2017). https://doi.org/10.1007/s10529-017-2366-6
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DOI: https://doi.org/10.1007/s10529-017-2366-6