Abstract
Objectives
To explore the effects of the competitive endogenous RNA (ceRNA) network between TP53INP1 and E-cadherin on the invasion and migration of glioma.
Results
TP53INP1 and E-cadherin mRNA and protein were significantly overexpressed in normal brain tissues compared with glioma tissue specimens and correlated with the grades of glioma negatively. The expression of TP53INP1 and E-cadherin were correlated positively. Patients with higher TP53INP1 or E-cadherin expression had longer overall survival. Moreover, TP53INP1 3′-UTR inhibited glioma cell proliferation, invasion and proliferation; Furthermore, the 3′-UTRs of TP53INP1 and E-cadherin harboured seven identical miRNAs binding sites, and TP53INP1 3′-UTR could increase the expression of E-cadherin and decrease the expression of vimentin thus repressing the epithelial-mesenchymal transition (EMT). However, the coding sequence of TP53INP1 could not increase the expression of E-cadherin and the inhibitory effect on EMT of TP53INP1 3′-UTR was reversed by the siRNA against Dicer.
Conclusions
TP53INP1 3′-UTR could inhibit the EMT, thus hindering the migration and invasion of glioma via acting as a ceRNA for E-cadherin.
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Change history
13 September 2023
This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1007/s10529-023-03431-6
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Acknowledgments
We thank Prof. Wu for critically reviewing this manuscript.
Supporting information
Supplementary Table 1—Primer sequences used for the construction of the TP53INP1-3’-UTR and TP53INP1-CDS, and primer sequences for TP53INP1 and E-cadherin qRT-PCR.
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Wang, Y., Lin, G. RETRACTED ARTICLE: TP53INP1 3′-UTR functions as a ceRNA in repressing the metastasis of glioma cells by regulating miRNA activity. Biotechnol Lett 38, 1699–1707 (2016). https://doi.org/10.1007/s10529-016-2159-3
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DOI: https://doi.org/10.1007/s10529-016-2159-3