Abstract
Objective
To analyze the involvement of the octadecanoic (OCDA) pathway in the accumulation of sanguinarine induced by yeast extract (YE) in cell suspension cultures of Argemone mexicana (Papaveraceae).
Results
Exposure to YE promoted sanguinarine accumulation. This was not observed when they were exposed to methyl jasmonate (MeJa). Use of diethyldithiocarbamic acid (DIECA), an inhibitor of the OCDA pathway, resulted in partial impairment of this response. Exogenous application of MeJa did not reverse this effect in DIECA-exposed cultures. qRT-PCR revealed that the accumulation of transcripts corresponding to the berberine bridge enzyme gene, which was induced by YE exposure, was blocked by OCDA pathway and reversed by exogenous MeJa. Interestingly, this response pattern could not be observed on dihydrobenzophenanthridine oxidase enzyme activity, which was promoted by YE, but unaffected by either OCDA or MeJa.
Conclusion
Results suggest partial involvement of OCDA pathway in this response.
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Acknowledgments
This work was supported by CONACYT (National Council for Science and Technology, Mexico), Grant CB-2012-0181800.
Supporting information
Supplementary Figure 1. Aspect of the A. mexicana AmMiF cell suspensions exposed to different doses of methyl jasmonate (MeJa) or yeast extract (YE) for 24 h. Cultures were treated as described in the Materials and methods section. Controls were exposed to water (for YE) or 10 % ethanol (for MeJa). Figures on top of photographs correspond to the concentration applied in μM for MeJa or mg l−1 for YE.
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Cecilia Guízar-González and Miriam Monforte-González have contributed equally to this work.
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Guízar-González, C., Monforte-González, M. & Vázquez-Flota, F. Yeast extract induction of sanguinarine biosynthesis is partially dependent on the octadecanoic acid pathway in cell cultures of Argemone mexicana L., the Mexican poppy. Biotechnol Lett 38, 1237–1242 (2016). https://doi.org/10.1007/s10529-016-2095-2
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DOI: https://doi.org/10.1007/s10529-016-2095-2