Abstract
Objectives
To produce and characterize novel laccases with ethanol tolerance from Trametes versicolor using agriculture by-products as energy source.
Results
Trametes versicolor 1017 produces two laccase isoenzymes with a total activity of 10 U ml−1 within 8 days when using wheat bran and peanut powder as energy sources in liquid culture medium. A novel isoenzyme, named Tvlac, was identified, purified and characterized. Its optimum pH and temperature were from 4.5 to 5 and 55 to 60 °C, respectively. Its activity was stimulated by ethanol at 10 % (v/v) which increased the V 0.
Conclusions
The biochemical properties of Tvlac substantiate the potential of this enzyme for applications under an aqueous ethanol mixture environment.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31300044), Scientific Research Foundation for Returned Scholars, Ministry of Education of China; the Natural Science Foundation of the Department of Education of Anhui Province (KJ2013A022); the Introduction Project of Academic and Technology Leaders in Anhui University (32030066) and the Innovative Research Team Program of 211 Project in Anhui University.
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Lei Chen and Xiaoming Yi have contributed equally to this work.
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Chen, L., Yi, X., Deng, F. et al. A novel ethanol-tolerant laccase, Tvlac, from Trametes versicolor . Biotechnol Lett 38, 471–476 (2016). https://doi.org/10.1007/s10529-015-1994-y
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DOI: https://doi.org/10.1007/s10529-015-1994-y