Abstract
Objectives
To evaluate the effects of 12 biotic and abiotic elicitors for increasing the production of plumbagin in Plumbago indica root cultures.
Results
Most elicitors showed minimal effects on the root dry weight, except for 250 mg chitosan l−1 and 10 mM l-alanine that markedly decreased root biomass by about 40 % compared to the untreated root cultures (5 g l−1). Treatments with 100 µM AgNO3 significantly increased intracellular plumbagin production by up to 7.6 mg g−1 DW that was 4-fold more than the untreated root cultures (1.9 mg g−1 DW). In contrast, treatments with 150 mg chitosan l−1, 5 mM l-alanine, and 50 µM 1-naphthol significantly enhanced the extracellular secretion of plumbagin by up to 10.6, 6.9, and 5.7 mg g−1 DW, respectively, and increased the overall production of plumbagin by up to 12.5, 12.5, and 9.4 mg g−1 DW, respectively.
Conclusions
Chitosan (150 mg l−1), l-alanine (5 mM), and 1-naphthol (50 µM) were the best elicitors to enhance plumbagin production in P. indica root cultures.
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Acknowledgments
The authors wish to thank Prince of Songkla University for a financial support in this research. The authors would also like to acknowledge Dr. Brian Hodgson for assistance with the English.
Supporting information
Supplementary Table 1—Elicitor stock solution preparation and the types and concentrations used.
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Jaisi, A., Panichayupakaranant, P. Increased production of plumbagin in Plumbago indica root cultures by biotic and abiotic elicitors. Biotechnol Lett 38, 351–355 (2016). https://doi.org/10.1007/s10529-015-1969-z
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DOI: https://doi.org/10.1007/s10529-015-1969-z