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Conditioned medium from mesenchymal stem cells enhances the migration of hepatoma cells through CXCR4 up-regulation and F-actin remodeling

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Abstract

Interactions between tumors and mesenchymal stem cells (MSCs) can regulate cancer cell behavior and cancer progression. Rat bone marrow-derived MSCs (rMSCs) were isolated and purified by Percoll density gradient centrifugation. Conditioned media from rMSCs (MSC-CM) was prepared, and its role in cancer cell migration and the underlying molecular mechanism were investigated. MSC-CM increased the migration and up-regulated the expression of CXC chemokine receptor 4 (CXCR4) in rat hepatoma cells (CBRH-7919). F-actin remodeling was observed, and the Young’s modulus was decreased in CBRH-7919 cells. A CXCR4 inhibitor suppressed the MSC-CM-induced CXCR4 expression and migration, restored the decrease in the Young’s modulus and disrupted the formation of F-actin. MSC-CM thus promotes CBRH-7919 cell migration by lessening cell stiffness and increasing F-actin formation through up-regulation of CXCR4 expression. MSC-CM may therefore have a positive impact on cancer metastases and underlines a potential safety issue associated with clinical applications of MSCs.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 11032012, 11102240, and 11272365), the 111 Project (No. 06023), the Visiting Scholar Foundation of Key Laboratory of Biorheological Science and Technology (Chongqing University), the Ministry of Education of China (No. CQKLBST-2012-008), and the Research Fund for the Doctoral Program of Higher Education of China (No. 20130191110029).

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Authors and Affiliations

  1. Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, 400044, People’s Republic of China

    Xiaoming Li, Qing Luo, Jinghui Sun & Guanbin Song

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  1. Xiaoming Li
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  2. Qing Luo
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  3. Jinghui Sun
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  4. Guanbin Song
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Correspondence to Guanbin Song.

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Li, X., Luo, Q., Sun, J. et al. Conditioned medium from mesenchymal stem cells enhances the migration of hepatoma cells through CXCR4 up-regulation and F-actin remodeling. Biotechnol Lett 37, 511–521 (2015). https://doi.org/10.1007/s10529-014-1710-3

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  • DOI: https://doi.org/10.1007/s10529-014-1710-3

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