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Upstream and downstream processing of recombinant IgA

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Abstract

Immunoglobulin A (IgA) is the most abundant antibody class in the human body and has a unique role in mediating immunity. The ever-increasing knowledge about the potential of IgAs has renewed interest in this antibody class for therapeutic use against a variety of infectious and malignant diseases, and as a preventive agent for mucosal pathogens. Despite the considerable therapeutic potential of IgA the exploration thereof has often been hampered due to difficulties in producing and purifying desired quantities. Large amounts of pure IgA will be required for in vivo studies. This work reviews current achievements and bottlenecks in upstream and downstream processing of recombinant IgA from a biotechnological point of view. We also highlight recent accomplishments with diverse expression systems and presents different affinity techniques for the capture of recombinant IgA to compare their purification potential.

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Authors and Affiliations

  1. Department of Biotechnology, Vienna Institute of BioTechnology, University of Natural Resources and Life Sciences, Muthgasse 11, 1190, Vienna, Austria

    David Reinhart & Renate Kunert

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  1. David Reinhart
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  2. Renate Kunert
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Correspondence to David Reinhart.

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Reinhart, D., Kunert, R. Upstream and downstream processing of recombinant IgA. Biotechnol Lett 37, 241–251 (2015). https://doi.org/10.1007/s10529-014-1686-z

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  • DOI: https://doi.org/10.1007/s10529-014-1686-z

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