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Probing the bioethanol production potential of Scheffersomyces (Pichia) stipitis using validated genome-scale model

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Abstract

A detailed in silico analysis of different strategies for enhancement of bioethanol production by Scheffersomyces stipitis, = Pichia stipitis, using validated genome-scale metabolic model is presented. Glucose inhibition on xylose uptake is dominant in S. stipitis which makes fed-batch fermentation more effective for higher sugar concentrations. Bioethanol production potential of S. stipitis can be improved by growth media modification by introducing certain amino acids in small quantities. Slower sugar uptake by S. stipitis can be overcome by community-culture with recombinant Escherichia coli strain ZSC113, which has a higher xylose uptake rate. Ethanol yield and productivity of community-culture can be further enhanced by genetic modification of E. coli strain ZSC113.

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Abbreviations

A :

Matrix of stoichiometric coefficients

a :

Specific interfacial air–liquid area, m−1

E :

Ethanol concentration g l−1

e:

Ethanol

EC:

Escherichia coli

F :

Feed rate l h−1

G :

Glucose concentration g l−1

G f :

Glucose concentration in the feed g l−1

g:

Glucose

K :

Half-saturation constant for substrate uptake g l−1

K ie :

Ethanol inhibition constant for substrate uptake g l−1

K igz :

Glucose inhibition constant for xylose uptake g l−1

k L :

O2 mass transfer coefficient m h−1

O :

Dissolved O2 concentration g l−1

o:

O2

SS:

Scheffersomyces stipitis

V :

Volume l

v :

Vector of reaction and exchange fluxes

w :

Vector of weights with contribution of flux to cell mass formation

X :

Cell mass concentration g l−1

Z :

Xylose concentration g l−1

z:

Xylose

Z f :

Xylose concentration in the feed g l−1

µ :

Specific growth rate h−1

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Authors and Affiliations

  1. Department of Chemical Engineering, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India

    Lisha K. Parambil & Debasis Sarkar

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  1. Lisha K. Parambil
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  2. Debasis Sarkar
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Correspondence to Debasis Sarkar.

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Parambil, L.K., Sarkar, D. Probing the bioethanol production potential of Scheffersomyces (Pichia) stipitis using validated genome-scale model. Biotechnol Lett 36, 2443–2451 (2014). https://doi.org/10.1007/s10529-014-1629-8

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  • DOI: https://doi.org/10.1007/s10529-014-1629-8

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