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Non-toxic and efficient DNA extractions for soybean leaf and seed chips for high-throughput and large-scale genotyping

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Abstract

In applied soybean (Glycine max L.) breeding programs, marker-assisted selection has become a necessity to select value-added quantitative trait loci. The goal of this work was to improve marker-assisted selection workflow by developing a reliable, inexpensive, high-throughput DNA extraction protocol for soybean seed and leaf samples that does not generate hazardous waste. The DNA extraction protocol developed allows for the leverage of robust SNP genotyping platforms such as the Simple Probe Assay and KASPar v4.0 SNP Genotyping System to genotype thousands of seeds or leaves non-destructively in a single day with a 95 % success rate. This methodology makes it possible to run up to 150 SNP markers on the DNA extracted from a single seed chip or leaf sample.

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Acknowledgments

This research was funded by the United Soybean Board Fellowship and the University of Georgia Graduate School Innovative and Interdisciplinary Research Grant.

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Institute for Plant Breeding, Genetics & Genomics, The University of Georgia, Athens, GA, 30602, USA

    Zachary King, Jonathan Serrano, H. Roger Boerma & Zenglu Li

Authors
  1. Zachary King
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  2. Jonathan Serrano
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  3. H. Roger Boerma
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  4. Zenglu Li
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Correspondence to Zachary King.

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King, Z., Serrano, J., Roger Boerma, H. et al. Non-toxic and efficient DNA extractions for soybean leaf and seed chips for high-throughput and large-scale genotyping. Biotechnol Lett 36, 1875–1879 (2014). https://doi.org/10.1007/s10529-014-1548-8

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  • DOI: https://doi.org/10.1007/s10529-014-1548-8

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