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A new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. Anaheim

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Abstract

Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflected in tissue bleaching and reduced transcript levels. The silencing was stable, reaching the flowers and fruits, and was observed throughout the life cycle of the plants. Additionally, the silencing of the NPR1 gene was efficient in both N. benthamiana and Capsicum annuum. After silencing, the plants’ viral symptoms increased to levels similar to those seen in wild-type plants. These results suggest that NPR1 plays a role in the compatible interactions of EuMV-YP N. benthamiana and EuMV-C. annum var. anaheim.

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Acknowledgments

We thank CONACyT for Grant # 106105 and for doctoral scholarship # 14557, which was awarded to H.J. Villanueva-Alonzo. We also thank Dr. Raúl Díaz Plaza at INIFAP for providing the manual bombardment gun.

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Authors and Affiliations

  1. Unidad de Bioquimica y Biologia Molecular de Plantas, Centro de Investigacion Cientifica de Yucatan AC, Calle 43 No. 130, Chuburna de Hidalgo, C.P. 97200, Merida, Yucatan, Mexico

    Hernan J. Villanueva-Alonzo, Rosa Y. Us-Camas, Luisa A. López-Ochoa, Yereni Minero-García & Oscar A. Moreno-Valenzuela

  2. Department of Plant Biology, North Caroline State University, 362 B Partners III. Centennial Campus, 7612, Raleigh, USA

    Dominique Robertson & Orlene Guerra-Peraza

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  1. Hernan J. Villanueva-Alonzo
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  2. Rosa Y. Us-Camas
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  3. Luisa A. López-Ochoa
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  4. Dominique Robertson
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  5. Orlene Guerra-Peraza
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  6. Yereni Minero-García
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  7. Oscar A. Moreno-Valenzuela
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Correspondence to Oscar A. Moreno-Valenzuela.

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Villanueva-Alonzo, H.J., Us-Camas, R.Y., López-Ochoa, L.A. et al. A new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. Anaheim. Biotechnol Lett 35, 811–823 (2013). https://doi.org/10.1007/s10529-013-1146-1

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  • DOI: https://doi.org/10.1007/s10529-013-1146-1

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