Abstract
Common buckwheat (Fagopyrum esculentum Moench) is rich in phenolic compounds and may be useful for the treatment of metabolic syndrome in humans. To improve the production of rutin in buckwheat, we overexpressed the flavonol-specific transcription factor, AtMYB12 using Agrobacterium rhizogenes into hairy root culture systems. This induced the expression of flavonoid biosynthetic genes encoding phenylalanine ammonia lyase, cinnamate 4-hydroxylase, 4-coumarate:CoA ligase, chalcone synthase, chalcone isomerase, flavone 3-hydroxylase, flavonoid 3′-hydroxylase, and flavonol synthase. This led to the accumulation of rutin in buckwheat hairy roots up to 0.9 mg/g dry wt. PAP1 expression, however, did not correlate with the production of rutin.
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References
Borevitz JO, Xia Y, Blount J, Dixon RA, Lamb C (2000) Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell 12:2383–2394
Du H, Zhang L, Liu L, Tang X-F, Yang W-J, Wu Y-M, Huang Y-B, Tang Y-X (2009) Biochemical and molecular characterization of plant MYB transcription factor family. Biochemistry 74:1–11
Gonzalez A, Zhao M, Leavitt JM, Lloyd AM (2008) Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings. Plant J 53:814–827
Hinneburg I, Neubert RHH (2004) Influence of extraction parameters on the phytochemical characteristics of extracts from buckwheat (Fagopyrum esculentum) herb. J Agric Food Chem 53:3–7
Kalinova J, Triska J, Vrchotova N (2006) Distribution of vitamin E, squalene, epicatechin, and rutin in common buckwheat plants (Fagopyrum esculentum Moench). J Agric Food Chem 54:5330–5335
Karimi M, Inzé D, Depicker A (2002) GATEWAY™ vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci 7:193–195
Kreft S, Strukelj B, Gaberscik A, Kreft I (2002) Rutin in buckwheat herbs grown at different UV-B radiation levels: comparison of two UV spectrophotometric and an HPLC method. J Exp Bot 53:1801–1804
Lepiniec L, Debeaujon I, Routaboul J-M, Baudry A, Pourcel L, Nesi N, Caboche M (2006) Genetics and biochemistry of seed flavonoids. Annu Rev Plant Biol 57:405–430
Li S-q, Zhang QH (2001) Advances in the development of functional foods from buckwheat. Crit Rev Food Sci Nutr 41:451–464
Li X, Park NI, Xu H, Woo S-H, Park CH, Park SU (2010) Differential expression of flavonoid biosynthesis genes and accumulation of phenolic compounds in common buckwheat (Fagopyrum esculentum). J Agric Food Chem 58:12176–12181
Luo J, Butelli E, Hill L, Parr A, Niggeweg R, Bailey P, Weisshaar B, Martin C (2008) AtMYB12 regulates caffeoyl quinic acid and flavonol synthesis in tomato: expression in fruit results in very high levels of both types of polyphenol. Plant J 56:316–326
Mehrtens F, Kranz H, Bednarek P, Weisshaar B (2005) The arabidopsis transcription factor MYB12 is a flavonol-specific regulator of phenylpropanoid biosynthesis. Plant Physiol 138:1083–1096
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Panchal SK, Poudyal H, Arumugam TV, Brown L (2011) Rutin attenuates metabolic changes, nonalcoholic steatohepatitis, and cardiovascular remodeling in high-carbohydrate, high-fat diet-fed rats. J Nutr 141:1062–1069
Park N, Xu H, Li X, Kim S-J, Park S (2011) Enhancement of flavone levels through overexpression of chalcone isomerase in hairy root cultures of Scutellaria baicalensis. Funct Integr Genomics 11:491–496
Timotijevic GS, Milisavljevic MD, Radovic SR, Konstantinovic MM, Maksimovic VR (2010) Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat. J Plant Physiol 167:61–68
Tohge T, Nishiyama Y, Hirai MY, Yano M, Nakajima J-i, Awazuhara M, Inoue E, Takahashi H, Goodenowe DB, Kitayama M, Noji M, Yamazaki M, Saito K (2005) Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor. Plant J 42:218–235
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This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A091220).
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Park, N.I., Li, X., Thwe, A.A. et al. Enhancement of rutin in Fagopyrum esculentum hairy root cultures by the Arabidopsis transcription factor AtMYB12. Biotechnol Lett 34, 577–583 (2012). https://doi.org/10.1007/s10529-011-0807-1
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DOI: https://doi.org/10.1007/s10529-011-0807-1