Abstract
Rab GTPase is required for vesicle–vacuolar fusion during the vacuolar biogenesis in fungi. Rab GTPase-encoding gene, pcvA, was cloned from Penicillium chrysogenum: it contained five introns and its predicted protein contained the conserved Rab GTPase domain involved in GTP-binding and hydrolysis. Over-expression of pcvA significantly stimulated the vesicle–vacuolar fusion but repressed the conidiation and decreased conidial tolerance against thermal stress. Penicillin production was decreased in the pcvA over-expressed strain suggesting that pcvA is involved in vesicle–vacuolar fusion participates in the penicillin biosynthesis in P. chrysogenum.
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Acknowledgments
We are grateful to Prof. Hua Xiang (Institute of Microbiology, CAS) for providing the fluorescence microscope. We thank Prof. Rongxiang Fang (Institute of Microbiology, CAS) for providing the plasmid pCAMBIA1302-LX-1. This work was supported by grants from the Ministry of Science and Technology of China (2009CB118905), the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KSCX2-EW-J-6) and the Ministry of Science and Technology of China (Grant No. 2010ZX09401-403).
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Xu, X., Yang, J., An, Y. et al. Over-expression of pcvA involved in vesicle–vacuolar fusion affects the conidiation and penicillin production in Penicillium chrysogenum . Biotechnol Lett 34, 519–526 (2012). https://doi.org/10.1007/s10529-011-0792-4
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DOI: https://doi.org/10.1007/s10529-011-0792-4