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Plant growth-promotion (PGP) activities and molecular characterization of rhizobacterial strains isolated from soybean (Glycine max L. Merril) plants against charcoal rot pathogen, Macrophomina phaseolina

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Abstract

Charcoal rot disease, caused by the fungus Macrophomina phaseolina, leads to significant yield losses of soybean crops. One strategy to control charcoal rot is the use of antagonistic, root-colonizing bacteria. Rhizobacteria A5F and FPT721 and Pseudomonas sp. strain GRP3 were characterized for their plant growth-promotion activities against the pathogen. Rhizobacterium FPT721 exhibited higher antagonistic activity against the pathogen on dual plate assay compared to strain A5F and GRP3. FPT721 and GRP3 gave decreased disease intensity in terms of average number of pathogen-infested plants. Lipoxygenase (LOX), phenylalanine ammonia-lyase (PAL), and peroxidase (POD) activities were estimated in extracts of plants grown from seeds that were treated with rhizobacteria, and inoculated with spore suspension of M. phaseolina. The activity of these enzymes after challenge with the test pathogen increased. Strains FPT721 and GRP3 exhibited maximum increases in LOX, PAL and POD activity (U mg−1 fresh leaf wt) compared to strain A5F.

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Acknowledgement

This research was supported by grant No. SR/FT/L-03/2006 to DKC from SERC division of Department of Science & Technology (DST), Govt. of India, New Delhi, India. The fungus, M. phaseolina and seeds of soybean were kindly provided by Dr. Mahaveer Sharma, from the National Research Centre for Soybean (NRCS), Indore (M.P.), India. Author is grateful to Prof. B.N. Johri and Dr. Anil Prakash, Department of Biotechnology, BU, Bhopal for their kind support during experiments. Mr. Ankit Kumar is gratefully acknowledged for providing a bacterial culture A5F for experiments. A kind support of Prof. Shakti Baijal, Dean, FASC, MITS, is gratefully acknowledged.

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  1. Department of Science, Faculty of Arts, Science and Commerce (FASC), Mody Institute of Technology & Science (MITS), Lakshmangarh, Sikar, 332311, Rajasthan, India

    D. K. Choudhary

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  1. D. K. Choudhary
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Correspondence to D. K. Choudhary.

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Choudhary, D.K. Plant growth-promotion (PGP) activities and molecular characterization of rhizobacterial strains isolated from soybean (Glycine max L. Merril) plants against charcoal rot pathogen, Macrophomina phaseolina . Biotechnol Lett 33, 2287–2295 (2011). https://doi.org/10.1007/s10529-011-0699-0

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