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Secretion of biologically-active human interferon-β by Bacillus subtilis

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Abstract

Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1.5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.

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Acknowledgments

This work is the subproject, ‘Development of a Technology for Creation of a Host Cell’ included within the industrial technology project, ‘Development of a Generic Technology for Production Process Starting Productive Function’ of the Ministry of Economy, Trade and Industry, entrusted by the New Energy and Industrial Technology Development Organization (NEDO), Japan.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8572, Japan

    Hiroshi Kakeshita & Kouji Nakamura

  2. Biological Science Laboratories, Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-Gun, Tochigi, 321-3497, Japan

    Hiroshi Kakeshita, Yasushi Kageyama, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara & Katsuya Ozaki

Authors
  1. Hiroshi Kakeshita
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  2. Yasushi Kageyama
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  3. Keiji Endo
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  4. Masatoshi Tohata
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  5. Katsutoshi Ara
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  6. Katsuya Ozaki
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  7. Kouji Nakamura
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Correspondence to Hiroshi Kakeshita.

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Kakeshita, H., Kageyama, Y., Endo, K. et al. Secretion of biologically-active human interferon-β by Bacillus subtilis . Biotechnol Lett 33, 1847–1852 (2011). https://doi.org/10.1007/s10529-011-0636-2

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  • DOI: https://doi.org/10.1007/s10529-011-0636-2

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