Abstract
Patatin-like phospholipases (PNPLAs) comprise a protein family whose members contain a conserved patatin-like domain with lipid acyl hydrolase activity. The cloning and characterization of full-length cDNAs of PNPLA3 and PNPLA4 in pigs is reported and, for the first time, an alternative splicing variant of porcine PNPLA4, which skips exon 5 of the normal transcript, was identified. Subsequently, tissue expression analysis by quantitative PCR indicated that porcine PNPLA3 was mainly expressed in both adipose and liver whereas porcine PNPLA4 was abundant in liver. Porcine PNPLA3 and PNPLA4 were assigned respectively to chromosome 5p11-p15 and Xp24.
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Acknowledgments
We are grateful to Dr. Martine Yerle and Denis Milan (INRA, Castanet-Tolosan, France) for kindly providing the RH panel. This work was supported by the grants from Major Projects of National Science and Technology (2009ZX08009-151B), 973 Programme (2006CB102100), 863 Programme (2008AA10Z134, 2006AA10Z140), High Education Doctorial Subject Research Programme (20060504016), General Programme (30771585) and Key Programe of National Natural Science Foundation of China.
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Zhilong Chen and Xin Gao contributed equally to this work.
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Chen, Z., Gao, X., Lei, T. et al. Molecular characterization, expression and chromosomal localization of porcine PNPLA3 and PNPLA4 . Biotechnol Lett 33, 1327–1337 (2011). https://doi.org/10.1007/s10529-011-0591-y
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DOI: https://doi.org/10.1007/s10529-011-0591-y