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Molecular characterization, tissue expression profile and SNP analysis of the porcine NR1H4 gene

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Abstract

Nuclear Receptor subfamily 1, group H, member 4 (NR1H4) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. In this study, we cloned the full coding region of NR1H4 gene from porcine Longissimus dorsi by Rapid amplification of cDNA end (RACE). Results indicated that the open reading frame of NR1H4 covered 1461 bp encoding 486 amino acid residues and the deduced amino acid sequence was 91–94 % identical to that of Homo sapiens, Bos taurus, Macaca mulatta, Gorilla gorilla, and Ovis aries. Bioinformatic analysis indicated that NR1H4 contained 31 phosphorylation sites with 14 serine, 6 threonine and 11 tyrosine. One single nucleotide polymorphism (SNP) was detected by PCR–RFLP in 3′ untranslated region of exon 9 (NR1H4) and the allele frequency analysis showed that A allele frequency was low among 396 pigs from five breeds. The NR1H4 mRNA expression pattern showed that NR1H4 gene was expressed highly in live and Longissimus dorsi. This work provided an important experimental basis for further research on mechanism of lipid metabolism and fat deposition in pigs.

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Acknowledgments

This study was supported by the National High Technology Research and Development Program of China (2011AA100304), the Provincial Natural Science Foundation of Hunan (13JJ1021) and A Foundation for the Author of National Excellent Doctoral Dissertation of PR China (200972).

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Authors and Affiliations

  1. College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, People’s Republic of China

    Hu Yang, Jun Jiang, Jun He, Changqing He & Haiming Ma

  2. College of Life Sciences and Resource Environment, Yichun University, Yichun, 336000, People’s Republic of China

    Hu Yang & Xingli Xu

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  1. Hu Yang
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  2. Jun Jiang
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  3. Xingli Xu
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  4. Jun He
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Correspondence to Haiming Ma.

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Yang, H., Jiang, J., Xu, X. et al. Molecular characterization, tissue expression profile and SNP analysis of the porcine NR1H4 gene. Mol Biol Rep 41, 7009–7014 (2014). https://doi.org/10.1007/s11033-014-3588-5

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  • DOI: https://doi.org/10.1007/s11033-014-3588-5

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