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Enhanced thermotolerance for ethanol fermentation of Saccharomyces cerevisiae strain by overexpression of the gene coding for trehalose-6-phosphate synthase

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Abstract

The effect of overexpression of the trehalose-6-phosphate (T6P) synthase gene (TPS1) on ethanol fermentation of Saccharomyces cerevisiae has been studied at 30 and 38°C. The activity of T6P synthase and the accumulation of trehalose during ethanol fermentation were significantly improved by overexpression of TPS1, and especially at 38°C. Ethanol produced by transformants with and without TPS1 gene overexpression at 38°C was approx. 60 and 37 g/l, respectively. The fermentation efficiency of transformants with TPS1 gene overexpression at 38°C was similar to that at 30°C. The critical growth temperature was increased from 36 to 42°C by TPS1 gene overexpression. These results indicated that overexpression of the TPS1 gene had a beneficial effect on the fermentation capacity of the title yeast strain at high temperatures.

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Authors and Affiliations

  1. Graduate School of Science and Technology, Kumamoto University, Kurokami 2-39-1, Kumamoto, 860-8555, Japan

    Ming-Zhe An, Kanako Mitsumasu, Morimura Shigeru & Kida Kenji

  2. College of Engineering, Peking University, Beijing, 100871, China

    Yue-Qin Tang & Ze-Shen Liu

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  1. Ming-Zhe An
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  2. Yue-Qin Tang
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  3. Kanako Mitsumasu
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  4. Ze-Shen Liu
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  5. Morimura Shigeru
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  6. Kida Kenji
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Correspondence to Yue-Qin Tang.

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An, MZ., Tang, YQ., Mitsumasu, K. et al. Enhanced thermotolerance for ethanol fermentation of Saccharomyces cerevisiae strain by overexpression of the gene coding for trehalose-6-phosphate synthase. Biotechnol Lett 33, 1367–1374 (2011). https://doi.org/10.1007/s10529-011-0576-x

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  • DOI: https://doi.org/10.1007/s10529-011-0576-x

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