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A comparison between osteogenic differentiation of human unrestricted somatic stem cells and mesenchymal stem cells from bone marrow and adipose tissue

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Abstract

To evaluate the potential of three stem cells for cell therapy and tissue engineering applications, the biological behavior and osteogenic capacity of the newly introduced cord-blood-derived, unrestricted somatic stem cells (USSC) were compared with those of mesenchymal stem cells isolated from bone marrow (BM-MSC) and adipose tissue (AT-MSC). There was no significant difference between the rates of proliferation of the three stem cells. During osteogenic differentiation, alkaline phosphatase (ALP) activity peaked on day 7 in USSC compared to BM-MSC which showed the maximum value of ALP activity on day 14. However, BM-MSC had the highest ALP activity and mineralization during osteogenic induction. In addition, AT-MSC showed the lowest capacity for mineralization during differentiation and had the lowest ALP activity on days 7 and 14. Although AT-MSC expressed higher levels of collagen type I, osteonectin and BMP-2 in undifferentiated state, but these genes were expressed higher in BM-MSC during differentiation. BM-MSC also expressed higher levels of ALP, osteocalcin and Runx2 during induction. Taking together, BM-MSC showed the highest capacity for osteogenic differentiation and hold promising potential for bone tissue engineering and cell therapy applications.

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Authors and Affiliations

  1. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran

    Abbas Shafiee, Ehsan Seyedjafari, Naser Ahmadbeigi & Peyman Dinarvand

  2. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran

    Ehsan Seyedjafari & Nasser Ghaemi

  3. Hematology Department, Faculty of Medical Science, Tarbiat Modares University, 14115-111, Tehran, Iran

    Masoud Soleimani

  4. Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Peyman Dinarvand

Authors
  1. Abbas Shafiee
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  2. Ehsan Seyedjafari
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  3. Masoud Soleimani
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  4. Naser Ahmadbeigi
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  5. Peyman Dinarvand
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  6. Nasser Ghaemi
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Corresponding author

Correspondence to Masoud Soleimani.

Additional information

Abbas Shafiee and Ehsan Seyedjafari contributed equally to this work.

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Supplementary material 1 (DOC 32 kb)

Flow cytometry analysis of stem cells (TIFF 631 kb)

10529_2011_541_MOESM3_ESM.tif

Adipogenic (a–c) and chondrogenic (d–f) differentiation of stem cells after 21 days of in vitro culture in induction medium (TIFF 7373 kb)

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Shafiee, A., Seyedjafari, E., Soleimani, M. et al. A comparison between osteogenic differentiation of human unrestricted somatic stem cells and mesenchymal stem cells from bone marrow and adipose tissue. Biotechnol Lett 33, 1257–1264 (2011). https://doi.org/10.1007/s10529-011-0541-8

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  • DOI: https://doi.org/10.1007/s10529-011-0541-8

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