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New method to enhance ajmalicine production in Catharanthus roseus cell cultures based on the use of cyclodextrins

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Abstract

The joint use of cyclodextrins and methyljasmonate, when accompanied by a short exposure to UV, enhanced extracellular ajmalicine accumulation to 1040 ± 26.6 mg/l in suspension cultured cells of Catharanthus roseus. The success of this strategy is due to the use of cyclodextrins, which not only induce ajmalicine biosynthesis but also promote adduct formation. This removes ajmalicine from the medium, reduces feedback inhibition and ajmalicine degradation, and allows its accumulation in the culture medium at elevated concentrations.

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Acknowledgments

Almagro L. held a grant from the MICINN. We thank José Martínez Parra and Ana Belén Sabater-Jara for the identification of indole alkaloids and Mariana Sottomayor for providing alkaloid standards. This work has been partially supported both by (BIO BVA 07 01 0003) and II PCTRM 2007-10 (08799/PI/08).

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Authors and Affiliations

  1. Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, 30100, Murcia, Spain

    L. Almagro, A. J. López Perez & M. A. Pedreño

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  1. L. Almagro
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  2. A. J. López Perez
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  3. M. A. Pedreño
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Correspondence to M. A. Pedreño.

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Almagro, L., López Perez, A.J. & Pedreño, M.A. New method to enhance ajmalicine production in Catharanthus roseus cell cultures based on the use of cyclodextrins. Biotechnol Lett 33, 381–385 (2011). https://doi.org/10.1007/s10529-010-0430-6

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  • DOI: https://doi.org/10.1007/s10529-010-0430-6

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